VOLAB对PCR 实验室规划设计的基本要求.docx

深圳市创美实业是一家专业从事现代实验室系统工程整体技术咨询、实验室布局规划、实验室装修设计、VOLAB品牌实验室家具、实验台、通风柜、安全防爆柜生产安装及服务一体化的股份制公司。实验室设计 VOLAB 对 PCR 实验室规划设计的基本要求 PCR 实验室依据所使用的方法可分为试剂准备、标本制备、扩增、产物分析等三或四个区域。只是使用实时荧光 PCR 仪、 HIV 病毒载量测定仪的 PCR 实验室,有上述前面三个区域即可。各区域应完全独立分隔,不应有任何的空气直通。 Based on the methods used by PCR lab, the PCR laboratory can be divided into three or four rooms or areas for (i) reagent preparation, (ii) sample preparation, (iii) amplification area, and (iv) product analysis area. If the PCR laboratory only uses real-time fluorescence PCR instruments and /or instruments for HIV virus load, the PCR lab may have only the first three rooms or areas (reagent preparation, sample preparation, amplification). The rooms or areas of the PCR lab should be independently separated, and there is no air flow through the separated rooms or areas. 产物分析区或三个区域的最后一个区域(即扩增及产物分析区),空气流向应由室外向室内,可通过在室内设置通风橱、排风扇或其他排风系统达到空气流由室外向室内流动的要求。 For PCR product analysis area or last area of three areas (area for amplification and product analysis), direction of air flow shall be from outside to inside of the area by setting up fume hood, exhaust fan or other exhaust system in the area. PCR 实验室各区域仪器设备配备通常如下: The equipment configuration for PCR labs are as follows: 试剂准备区: Reagent preparation area 仪器设备主要应有加样器、冰箱、天平、低速离心机、混匀器、可移动紫外灯等。可使用超净工作台作为试剂配制操作台面。 The main equipment here should be: pipette, refrigerator, balance, low speed centrifuge, vortex mixer, mobile UV lamp, etc. It can use Laminar flow for the reagent preparation. 标本制备区 sample