鼻腔吸入IFNr治疗变应性鼻炎的实验研究（医学论文）.doc

鼻腔吸入IFNr治疗变应性鼻炎的实验研究（医学论文）.doc鼻腔吸入IFNr治疗变应性鼻炎的实验研究【摘要】探讨鼻腔吸入r•干扰素(IFNr)对变应性鼻炎(allergicrhinitis,AR)的防治作用。方法:采用卵清蛋白(OVA)、氢氧化铝建立大鼠AR模型。IFNr鼻腔吸入组(C组)与模型建立第2330天,每日1次鼻腔吸入IFNr12?gg,第31天取鼻腔灌洗液测定细胞成分,白介素(IL)4,IL5浓度,取血测定血浆IgE水平。设正常对照组(A组),AR模型磷酸盐缓冲液(PBS)处理组(B组)和丙酸倍***米松吸入组(D组),每组5只大鼠。结果:C组鼻腔灌洗液中的嗜酸性粒细胞(EOS)为(0・005±)X104/ml,明显低于E组的(±)X104/ml(P<0・01);C组鼻腔灌洗液中的IL4为(±)pg/mL,IL5为(±)pg/mL,均明显低于E组(P<);C组血浆中总IgE为(±)Mg/ml,OVA特异性IgE为(±)U/mL,明显低于E组(P<)。结论:鼻腔吸入IFNI•可以抑制AR鼠IL4和IL5的合成,抑制EOS在鼻腔内的炎性浸润及血浆中总IgE和OVA特异性IgE水平,因而,对AR具有一定的防治作用。【关键词】r干扰素鼻炎变应性雾化吸入治疗结果ATreatmentofallergicrhinitisbynebulizedinterferongamma[ABSTRACT]Objective:Toinvestigatetheefficacyandmechanismofnebulizedinterferongamma(IFNr)inthepreventionandtreatmentofallergicrhinitis・Methods:Ovalbumirl(OVA)absorbedtoaluminumhydroxidewasusedtoconstructtheallergicrhinitismodel(groupC),andnebulizedIFNr12?,andthecellularconstituents,levelsofIL4,IL5andIgEweredetermined・Thenormalcontrolgroup(groupA),theallergicrhirdticmodelgroup(groupB)andbeclomethasonedipropionategroup(groupD):Decreaseofeosinophils(±)X104/paredwithgroupB[(±)X104/ml,(P<)],andthelevelsofIL4(±?pg/mL)andIL5(±)parisonwithgroupB(P<).TheserumlevelsoftotalIgE(±)andovalbuminspecificIgE(±5・2U/mL