脑络通粉对局灶性脑缺血的保护作用的实验的分析.pdf

延边人学瞰学部慷I学任论史乏桷崾摘要目的通过动物试验研究,探i寸新药“脑络通粉”对人l’I鼠^6★I:r胁i缺帆模掣坝伤f门保护作川,验“腑络通粉对缺帆忭腑lfJ风的保护作川。方法通过IflL僻内线栓附断法缱移人鼠人脑·l,动瞅jIIj★I:忭腑缺m模J‰观察腑络通粉埘腑缺I缸人鼠的行为状念、腑缺衄lfIf髟!,腑鲰l纵形态学的作川。采川八道乍胖也菜仪观察腑络通粉住给约}jif后舯麻孵火m流动力学羊¨腑瞰流链、脑m骨附力的作川;采川:磷酸腺仟‘ADP)涛导法。川LBY-NJ,弘l『Ⅱ小扳聚凭仪,测定60s, 180s、300s m小板聚集强度,最人聚集骰度(MAl:采IfJ xsN一12掣体外m枪形成仪测定lflL抡的&度、泓莆和r蓖;动物均皮F注射肾1.-腺素(Adr). ,检测令血比耦度(商、Ifi、低切变二棼)。血浆比枯度.’}l:细胞瓜积(HCT):Hj 、#自动m凝分析仪进行凝m酶原时『HJ(PT)及部分凝虮活酶时洲(AP’rT)测定。":的喘气时删。结果研究结果表明:,kg组町明锰改薛造模大鼠脑缺血后的种经*#状(Jp<)、缩小腑缺血范嘲、抑制了神经细胞的绢理学改变。腑络通粉lI丁增加麻阱犬脑m流最【P|<、|p<、P<O.(J(II).降低脑血管阻力(P<、JP<)。∥kg、,kg、:i组削毓均埘模龌人鼠m浆I缸小板聚集j{订 1i川样度的抑制作刖(P<().05):∥妇组对人鼠仑血比粘度的高切, IfJ切、低切,}I:细胞压秘‘i模型对jl{{(Jp<l'.05、,,<、,'<):脑络通粉再刺醚组时人鼠体外血栓的长度,湿币年II f?霞、均l}彳|-HJj轻的降低作JfjtP<,|p<、尸<):,(AP|rT)时nU』l仃HJj移的延长作j|j(JI)<、Jf'<、.『'<,. ’;外时断火小鼠仔活时问fJ.}|Jj娃延长f1:『|J(JI)<I).05、,'<)。结论脑络通粉能够[Ii[辫腑循环,增加缺lfILI xI腑m流髓,对腑缺m仃t定的f垠护作用。关键词腑络通粉:^0tLr}腑缺m;晌血流:I}:舭i咀锊m力:约效学迁边J、学It学韶俩ll学恤论c[= 诞t掬嚷 Abstract objective T0 explore theprotectiVe e腑ctol-Naoluotong Powdcr INPl on ischcmia injuO。modcl ofrat.’roVerify theefkct ofZP on ischcmic stroke proteclion. Methods Focal cerebral ischemia modcl was made“a thethrcad—occluding method toexpIore theprotective ef&ctsofNP on the scorc ofneumIogical rate ofcercbral infarctionand histomorphology of cerebral ischemia efkcts of ZP on the bIood now龃d cercbral vaLscular resistance inthe anaesthctized dogⅥ’ere inVestigated inthe 8一channel ph)‘siology ADP—induced pldtelet aggregation w私fespectively measured 6y LBY-NJ—pIatelet aggregation intensity ofplatelctaggrcgalion and the maximaI aggregation intensily inthetime Of6(}。300s were thrombus weight and dry weight weredete呻ined by the extracorporeall XSN一12 typethrombopoiesis inst