急性低温再复温对大鼠心室肌膜电位和钾电流影响英文.doc

急性低温_再复温对大鼠心室肌膜电位和钾电流的影响_英文_ActaPhysiologicaSinica,June25,2008,60(3):311-319311+Effectsofacutecooling/rewarmingonmembranepotentialandKcurrentsinratventricularmyocytes1,21,221112LILong-Tian,ZHANGLi-Bin,SIYan-Li,XIAOFu-Cheng,LIDa,GAOShan,LIDong-Liang,1,*ZHOUShi-Sheng1LiaoningKeyLaboratoryofBiophysics,InstituteofBasicMedicalSciences,MedicalCollege,DalianUniversity,Dalian116622,2China;DepartmentofPhysiology,XinxiangMedicalCollege,Xinxiang453003,China+Abstract:Theeffectsofacutecooling/-cellpatch-clamptechniqueincurrent-andvoltage-+decreasedfrom25ºCto4ºC,thetransientoutwardcurrent(I)pletelyabolished,thesustainedoutwardKcurrent(I)at+60mVtoss+andtheinwardrectifierKcurrent(I)at-120mVweredepressedby(?)%and(?)%,respectively,ºCto36ºC,themembranepotentialexhibiteda+(36outof58),activationoftheATP-sensitiveK++(K)-inducedchangeinthemembranepotentialwasinhibitedbytheNa/K-ATPaseATPinhibitorouabain(100μmol/L),andtherewarming-elicitedactivationofKchannelswasinhibitedbytheproteinkinaseAinhibitorH-89ATP(100μmol/L).Moreover,decreaseofthetemperaturefrom25ºCto4º,significantcellshrinkagewithspotswasobservedsoonafterrewarming-induced+,ooling/:hypothermia;rewarming;potassiumchannels;membranepotentials;myocardium急性低温/再复温对大鼠心室肌膜电位和钾电流的影响1,21,2211121,*李龙天,张利彬,司艳丽,肖付诚,李达,高姗,李东亮,周士胜12辽宁省高校生物物理学重点实验室,大连大学医学研究中心,大连116622;新乡医学院生理学教研室,新乡453003摘要:本文旨在研究急性低温/再复