柳茶多糖的分离纯化及气相色谱-质谱分析.doc

柳茶多糖的分离纯化及气相色谱-质谱分析
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作者:石磊,张冬梅,齐洁,李建银,杨永建
【摘要】目的首次提取柳茶中的水溶性粗多糖并进行分离、纯化及组成分析。方法柳茶经热水提取、乙醇沉淀、Sevag法去蛋白、H2O2脱色,逆向流水透析得多糖精制品。将精多糖衍生化后运用气相色谱-质谱法对其化学成分进行分析。结果确定了柳茶中水溶性多糖分别由核糖、甘露糖、葡萄糖和半乳糖组成,其中以核糖、葡糖糖、半乳糖为主。结论该方法简便、灵敏,可以准确测定出柳茶多糖中所含有的各种单糖。
【关键词】柳茶多糖; 纯化; 气相色谱-质谱; 单糖
Abstract:ObjectiveTo isolate and purify the water-soluble haride from Liucha and analyze the position of liucha was extracted in hot water, then the haride in the filtrate was fractionally precipitated by alcohol,after that the proteins in the precipitated product were removed by Sevag reagent,hydrogen peroxide decolor, the hydrolysate of haride was dialyzed by the reverse flow to ge
t the fine product. Finally, the positions of the fine product after derivatization were examined by the gas chromatography-mass spectrometric harides posed of ribose,mannose,glucose and major ponents are ribose, galactose and method reported in this paper is simple,and can be used for analysis of harides hydrolyzed from Liucha haride.
Key words: Liucha haride; Purify; Gas chromatography-mass spectrometry; haride
柳茶为藏族民间常用药,以蔷薇科(Rosaceae)鲜卑花属(Sibiraea)植物窄叶鲜卑花Sibiraea angustata (Rehd.) Hand-Mazz. 和鲜卑花Sibiraea laecigata (L) (带果实的果枝)入药,主要用于消食导滞、疏散风热;据藏药文献记载,柳茶主治热病和疫病[1]。现藏族民间常作茶饮,治疗食后腹胀等诸多原因引起的消化不良,每每见效。柳茶