毛细管电泳法快速测定齐墩果酸片的含量.doc

毛细管电泳法快速测定齐墩果酸片的含量
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作者:黄雅燕,肖美添,叶静,汤须崇
【摘要】目的建立快速测定齐墩果酸片含量的方法。方法采用毛细管电泳法,未涂层弹性石英毛细管(75 μm×50 cm,有效长度40 cm);压力进样,压力: kPa;进样时间:5 s;分离电压:25 kV;柱温:25 ℃;检测波长:200 nm;运行缓冲液:含5%(体积分数)甲醇的20 mmol·L-1硼砂溶液(pH );运行时间:10 min。结果齐墩果酸的线性范围为 ~660 μg·mL-1(r= 7),%,% (n=6)。结论方法准确、快速、简便,适于齐墩果酸片的含量测定。
【关键词】齐墩果酸片毛细管电泳含量测定
Abstract:Objective To establish an HPCE method for rapid monitoring the content of oleanolic acid in oleanolic acid tablets. Methods Samples were analyzed on a fused and uncoated silic capillary of 75 μm×50 cm (40 cm of effective length). 20 mmol·L-1 of borax containing 5% methanol was used as running buffer. Samples were injected hydrodynamically (5 s, 3.
0 kPa) into the capillary. The applied voltage was 25 kV and the detection wavelength was set at 200 nm. The temperature was kept at 25 ℃. The running time was 10 min. Results The calibration curve was linear in the range of -660 μg·mL-1(r=) for oleanolic acid. The average recovery (n=6) was % with RSD %. Conclusion This method is accurate, rapid, and simple for the content determination of oleanolic acid.
Key words: oleanolic acid tablet; HPCE; content determination
齐墩果酸片具有护肝降酶、抗炎、强心利尿、抗肿瘤等作用,临床上除广泛应用于急、慢性肝炎的辅助治疗外,也用于配合肿瘤病人的手术、放疗、化疗,促进免疫功能恢复以及再生障碍性贫血、红斑狼疮等免疫缺陷性疾病。目前国内有许多厂家生产齐墩果酸片,生产上采用酸碱非水滴定法[1]测定其含量,但该方法终点不易掌握,专属性差,误差较大。也