鳖甲煎丸对腹膜透析相关腹膜纤维化的影响及 机制探讨.doc

鳖甲煎丸对腹膜透析相关腹膜纤维化的影响及 机制探讨


该论文来源于网络，本站转载的论文均是优质论文，供学****和研究使用，文中立场与本网站无关，版权和著作权归原作者所有，如有不愿意被转载的情况，请通知我们删除已转载的信息，如果需要分享，请保留本段说明。
　　[摘要] 目的 探討鳖甲煎丸对腹膜透析（PD）小鼠腹膜纤维化的抑制作用及可能机制。 方法 % mL每日腹腔注射一次，并于第3、5、 mg/kg腹腔注射造模; g/kg每日灌胃一次，干预6周后，测腹透超滤量，HE染色和Masson染色观察壁层腹膜形态改变及腹膜厚度;Western blot检测脏层腹膜组织N-cadherin、ZO-1的蛋白表达水平，及pAkt、总Akt的蛋白表达水平。 结果 腹膜透析（PD）组、鳖甲煎丸（BJJW）组与对照（Control）组比较，超滤量均显著下降（P<）;壁层腹膜均显著增厚（P<）。脏层腹膜ZO-1蛋白表达降低，N-cadherin蛋白表达升高，pAkt也明显升高（均P<）。BJJW组与PD组比较，超滤量明显改善（P<），壁层腹膜厚度降低（P<）;脏层腹膜ZO-1蛋白表达增加，N-cadherin蛋白表达下降，pAkt也明显下降（均P<）。 结论 鳖甲煎丸抑制腹膜纤维化，PI3K/Akt信号调节腹膜间皮细胞上皮-间质细胞转分化（EMT）是其机制之一。
　　[关键词] 鳖甲煎丸;腹膜透析;腹膜纤维化;上皮-间充质转分化;PI3K/Akt信号
　　[中图分类号] R285 [文献标识码] A [文章编号] 1673-9701（2020）09-0032-04
　　[Abstract] Objective To investigate the inhibitory effect of Biejiajian pill on peritoneal fibrosis in peritoneal dialysis（PD） mice and its possible mechanism. Methods ICR mice were injected intraperitoneally with % peritoneal dialysis solution mL once a day， combined with lipopolysaccharide mg/kg on the 3rd， 5th and 7th day to build model. Biejiajian pill g/kg were administrated daily by gavage. After 6 weeks of intervention， the volume of peritoneal ultrafiltration was measured， HE staining and Masson staining were used to observe the changes in peritoneal morphology and peritoneal thickness of the parietal peritoneum. Western blot was used to detect the expression levels of N-cadherin and ZO-1， pAkt and total Akt in visceral peritoneal tissue. Results Compared with that of the control group， the volume of peritoneal ultrafiltration in the peritoneal dialysis（PD） group and Biejiajian pill（BJJW） group was significantly lower（P<）， and the parietal peritoneum in PD group and BJJW group was significantly thickened（P<）. Visceral peritoneal ZO-1 protein expression was reduced， N-cadherin protein expression was increased， and pAkt was al