牡丹皮抗内毒素活性研究.doc

牡丹皮抗内***活性研究
陈益国1，2 曹红卫1，王宁1，郑江1
1．第三军医大学西南医院综合实验研究中心 邮政编码　400038.
2．江西省人民医院检验科，邮政编码　330006.
陈益国，医学博士，主要从事抗感染免疫研究，，；
郑江，通讯作者，博士生导师，主要从事抗感染免疫研究， , Tel:。
摘要
目的：利用生物传感器及纯化技术，对传统中药牡丹皮（Paeonia suffruticosa Andr, PSA）抗内***活性组分进行定向分离及体内外活性评价。方法：利用生物传感器、硅胶柱层析、聚酰***层析、离子交换-HPLC等技术对牡丹皮进行活性组分的定向分离。、对LPS和热灭活大肠杆菌攻击小鼠的保护实验，评价所得活性组分对内***的拮抗作用。结果 从牡丹皮水提取物中分离到一个与Lipid A具有较高结合活性的组分，-α，%，%，与对照组比较，均具有显著性差别。结论 从牡丹皮中分离到一个与Lipid A具有较高结合活性的组分，该组分在体内外对LPS均具有一定的拮抗作用。
关键词：中药，生物传感器，内***，牡丹皮，脓毒症
Anti-endotoxin bioactivities of the Paeonia suffruticosa Andr.
Chen yiguo1,2 ,Cao hongwei1, Wang ning1, and Zhengjiang1
1. Medical Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038
Clinical Labs, Peoples’ Hospital of Jiangxi Province, Nanchang 330006.
Abstract
Objective: The aim of our study is to isolate anti-LPS components from Paeonia suffruticosa Andr that can highly bind to Samonella Lipid A (Re595) in our preliminary experiment, then further study their bioactivity of anti-LPS in vitro and in vivo. Methods: (1) we isolated anti-LPS fractions from Paeonia suffruticosa Andr by many means which included biosensor, solvents abstraction, polyamide chromatography, silica gel colum chromatography and IEC- HPLC. (2) we investigated anti-LPS activities of t