rapid and accurate detection of pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrb gene论文.pdf

Diagnostic Microbiology and Infectious Disease 58 (2007) 53–58

Rapid and accurate detection of Pseudomonas aeruginosa by real-time
polymerase chain reaction with melting curve analysis targeting gyrB gene
Maiko Motoshimaa, Katsunori Yanagiharaa,4, Kazuko Fukushimaa, Junichi Matsudaa,
Kazuyuki Sugaharaa, Yochi Hirakataa, Yasuaki Yamadaa, Shigeru Kohnob,c, Shimeru Kamihiraa
aDepartment of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City, Nagasaki 852-8501, Japan
bSecond Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City, Nagasaki 852-8501, Japan
cCentral Diagnostic Laboratory, Nagasaki University Hospital, Nagasaki City, Nagasaki 852-8501, Japan
Received 10 July 2006; accepted 6 November 2006
Abstract
Laboratory detection of Pseudomonas spp., particularly Pseudomonas aeruginosa, is an important assay in the nosocomial control. The
study was designed firstly to establish a new assay-applied LightCycler polymerase chain reaction (PCR) technology with melting curve
analysis (MCA). A total of 224 Gram-negative isolates were