rapid detection and differentiation of the exfoliative toxin a-producing staphylococcus aureus strains based on eta prophage polymorphisms论文.pdf

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Diagnostic Microbiology and Infectious Disease 66 (2010) 248–252

Rapid detection and differentiation of the exfoliative toxin A-producing
Staphylococcus aureus strains based on ϕETA prophage polymorphisms
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Pavla Holochováa, Vladislava Růžičkováa, , Lucie Dostálováa, Roman Pantůčeka,
Petr Petrášb,JiříDoškař a
aFaculty of Science, Department of Genetics and Molecular Biology, Institute of Experimental Biology, Masaryk University, 611 37 Brno, Czech Republic
bReference Laboratory for Staphylococci, National Institute of Public Health, 100 42 Prague, Czech Republic
Received 9 June 2009; accepted 7 October 2009
Abstract
The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction
multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various ϕETA prophages of serogroup B responsible for
dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-posi