rapid detection of clostridium difficile in stool using the vidasrc. difficile toxin a ii assay论文.pdf

Diagnostic Microbiology and Infectious Disease
45 (2003) 117–121
Rapid detection of Clostridium difficile in stool using the
VIDASR C. difficile Toxin A II assay
. Lipsona* , G. Tortoraa, A. Temponea, . Fedorkob, . Spitzera
aClinical Microbiology Laboratory, Department of Laboratories, SUNY at Stony Brook, Stony Brook, NY, USA
bInfectious Diseases Laboratory, NIH, Bethesda, MD, USA
Received 29 August 2002; accepted 23 October 2002
A rapid laboratory diagnosis of Clostridium difficile-associated diarrhea (CDAD) is important in patient management and in the
administration of appropriate therapeutic modalities. The VIDASR C. difficile Toxin A II (CDA 2) assay (bioMerieux, Inc., Hazelwood, MO)
was compared with the cell culture cytotoxicity assay (CCA) for the rapid detection of C. difficile in stool from patients in whom C. difficile
infection was suspected. Thirty-eight consecutively collected CCA-positive stool specimens, and 33 CCA-negative stool specimens were
tested by the CDA 2 assay. Where appropriate, discordant specimens were repeated and/or tested by isolation utilizing cycloserine-cefoxitin