rapid detection of coinfections by trichomonas vaginalis, mycoplasma hominis, and ureaplasma urealyticum by a new multiplex polymerase chain reaction论文.pdf

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Diagnostic Microbiology and Infectious Disease 67 (2010) 30–36

Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma
hominis, and Ureaplasma urealyticum by a new multiplex
polymerase chain reaction
⁎
Nicia Diaza, Daniele Dessìa, Salvatore Dessoleb, Pier Luigi Fioria, Paola Rappellia,
aDepartment of Biomedical Sciences, Gynecology, and Obstetrics, University of Sassari, viale 43/B, 07100, Sassari, Italy
bDepartment of Pharmacology, Gynecology, and Obstetrics, University of Sassari, viale 43/B, 07100, Sassari, Italy
Received 31 July 2009; accepted 22 December 2009
Abstract
We developed a multiplex polymerase chain reaction (M-PCR) assay to simultaneously detect Trichomonas vaginalis, Mycoplasma
hominis, and Ureaplasma urealyticum. The test is extremely specific and has a sensitivity of 10 cells for T. vaginalis and U. urealyticum and
of 1 cell for M. hominis. The technique was validated on vaginal s