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rapid detection of coinfections by trichomonas vaginalis, mycoplasma hominis, and ureaplasma urealyticum by a new multiplex polymerase chain reaction论文.pdf


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rapid detection of coinfections by trichomonas vaginalis, mycoplasma hominis, and ureaplasma urealyticum by a new multiplex polymerase chain reaction论文.pdf
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Available online at www.sciencedirect.com
Diagnostic Microbiology and Infectious Disease 67 (2010) 30–36
www.elsevier.com/locate/diagmicrobio
Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma
hominis, and Ureaplasma urealyticum by a new multiplex
polymerase chain reaction

Nicia Diaza, Daniele Dessìa, Salvatore Dessoleb, Pier Luigi Fioria, Paola Rappellia,
aDepartment of Biomedical Sciences, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
bDepartment of Pharmacology, Gynecology, and Obstetrics, University of Sassari, viale S.Pietro 43/B, 07100, Sassari, Italy
Received 31 July 2009; accepted 22 December 2009
Abstract
We developed a multiplex polymerase chain reaction (M-PCR) assay to simultaneously detect Trichomonas vaginalis, Mycoplasma
hominis, and Ureaplasma urealyticum. The test is extremely specific and has a sensitivity of 10 cells for T. vaginalis and U. urealyticum and
of 1 cell for M. hominis. The technique was validated on vaginal s
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