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rapid detection of methicillin-susceptible and methicillin-resistant staphylococcus aureus directly from positive bactalert blood culture bottles using real-time polymerase chain reaction evaluation a论文.pdf


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rapid detection of methicillin-susceptible and methicillin-resistant staphylococcus aureus directly from positive bactalert blood culture bottles using real-time polymerase chain reaction evaluation a论文.pdf
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Available online at www.sciencedirect.com
Diagnostic Microbiology and Infectious Disease 61 (2008) 446–452
www.elsevier.com/locate/diagmicrobio
Antimicrobial Susceptibility Studies
Rapid detection of methicillin-susceptible and methicillin-resistant
Staphylococcus aureus directly from positive BacT/Alert® blood culture
bottles using real-time polymerase chain reaction: evaluation and
comparison of 4 DNA extraction methods

Graham Michael Hogg , James Patrick McKenna, Grace Ong
Department of Microbiology, Belfast Health and Social Care Trust, Kelvin Building, Belfast BT12 6BA, Northern Ireland, United Kingdom
Received 30 November 2007; accepted 22 March 2008
Abstract
Although the introduction of automated blood culture systems has dramatically reduced laboratory personnel bench time, 48 to 72 h is still
required for the identification of pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus
(MSSA) and the accurate determination of antimicrobial sensitivities for prompt optimal patient therapy and infect
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