rapid detection of methicillin-susceptible and methicillin-resistant staphylococcus aureus directly from positive bactalert blood culture bottles using real-time polymerase chain reaction evaluation a论文.pdf
Available online at Diagnostic Microbiology and Infectious Disease 61 (2008) 446–452
Antimicrobial Susceptibility Studies Rapid detection of methicillin-susceptible and methicillin-resistant Staphylococcus aureus directly from positive BacT/Alert® blood culture bottles using real-time polymerase chain reaction: evaluation and comparison of 4 DNA extraction methods ⁎ Graham Michael Hogg , James Patrick McKenna, Grace Ong Department of Microbiology, Belfast Health and Social Care Trust, Kelvin Building, Belfast BT12 6BA, Northern Ireland, United Kingdom Received 30 November 2007; accepted 22 March 2008 Abstract Although the introduction of automated blood culture systems has dramatically reduced laboratory personnel bench time, 48 to 72 h is still required for the identification of pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) and the accurate determination of antimicrobial sensitivities for prompt optimal patient therapy and infect
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