rapid differentiation between clinically relevant mycobacteria in microscopy positive clinical specimens and mycobacterial isolates by line probe assay论文.pdf

Diagnostic Microbiology and Infectious Disease
43 (2002) 297–302
Mycobacteriology
Rapid differentiation between clinically relevant mycobacteria in
microscopy positive clinical specimens and mycobacterial isolates by
line probe assay
Isik Somuncu Johansena,*, Bettina Lundgrenb, Jacob Pontoppidan Thyssena,
Vibeke Østergaard Thomsena
aInternational Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark
bDepartment of Clinical Microbiology, Hvidovre University Hospital, Copenhagen, Denmark
Received 14 January 2002; accepted 25 April 2002
Abstract
The Inno LiPA Mycobacteria assay, based on PCR amplification of the 16–23S rRNA spacer region of Mycobacterium species, has been
designed for identification of mycobacteria grown in culture media and discrimination between Mycobacterium tuberculosis complex,M.
avium, M. intracellulare, M. kansasii, M. gordonae, M. xenopi, scrofulaceum and M. chelonae group including M. abscessus. In order to
evaluate the system as a fast diagnostic tool, the assay was for the first time used directly