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rapid latex agglutination test for the serodiagnosis of human brucellosis论文.pdf


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rapid latex agglutination test for the serodiagnosis of human brucellosis论文.pdf
文档介绍:
Diagnostic Microbiology and Infectious Disease 57 (2007) 123–128
www.elsevier.com/locate/diagmicrobio
Rapid latex agglutination test for the serodiagnosis of human brucellosis
Theresia H. Abdoel, Henk L. Smits4
KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), 1105 AZ Amsterdam, The Netherlands
Received 16 February 2006; accepted 23 August 2006
Abstract
We developed and evaluated a user-friendly latex agglutination assay for the serodiagnosis of human brucellosis. The assay was obtained
by coating colored latex beads with Brucella lipopolysaccharides and drying of the activated beads onto white agglutination cards. Individual
cards were sealed in a protective foil to secure stability of the dried reagent and to obtain a test in a single assay format. The latex
agglutination assay is simply performed by suspending the dried latex reagent in a drop of serum and looking for macroscopic agglutination
of the latex beads by visual inspection. Results are obtained within 30 s after mixing the sample with the test reagent. The sensitivity of the
assay was determined to be 89.1% (95% confidence interval [CI], 76–96) for the initial serum samples collected from patients wi
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