rapid identification of fonsecaea by duplex polymerase chain reaction in isolates from patients with chromoblastomycosis论文.pdf

Diagnostic Microbiology and Infectious Disease 57 (2007) 267–272

Mycology
Rapid identification of Fonsecaea by duplex polymerase chain reaction
in isolates from patients with chromoblastomycosis
Taˆnia Sueli de Andradea,4, Arlete Emily Curya, Luiz Guilherme Martins de Castrob,
Mario Hiroyuki Hirataa, Rosario Dominguez Crespo Hirataa
aDepartamento de Ana´lises Clı´nicas e Toxicolo´gicas da Faculdade de Cieˆncias Farmaceˆuticas da Universidade de Sa˜o Paulo, Sa˜o Paulo, Brasil
bDivisa˜o de Dermatologia do Hospital das Clı´nicas da Faculdade de Medicina da Universidade de Sa˜o Paulo, Sa˜o Paulo, Brasil
Received 8 May 2006; accepted 29 August 2006
Abstract
Fonsecaea pedrosoi is the most common etiologic agent of chromoblastomycosis. F. pedrosoi and other dematiaceous fungi are usually
identified by morphologic studies. We have developed a duplex polymerase chain reaction (PCR) targeting the ribosomal DNA for rapid and
more specific identification of the genus Fonsecaea. DNA samples from 103 isolates of Fonsecaea species and other dematiaceous fungi
were amplified by PCR using universal and spec