rapid identification and specific quantification of staphylococcus epidermidis by 5′ nuclease real-time polymerase chain reaction with a minor groove binder probe论文.pdf

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Diagnostic Microbiology and Infectious Disease 60 (2008) 217–219

Notes
Rapid identification and specific quantification of Staphylococcus
epidermidis by 5′ nuclease real-time polymerase chain
reaction with a minor groove binder probe
⁎
Tadayuki Iwasea, , Sadayori Hoshinab, Keiko Sekia, Hitomi Shinjia,
Shogo Masudaa, Yoshimitsu Mizunoea
aDepartment of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan
bDepartment of Laboratory Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan
Received 20 April 2007; accepted 22 August 2007
Abstract
A species-specific quantitative detection method involving 5′ nuclease real-time polymerase chain reaction using a minor groove binder
probe that was designed from the sodA gene was developed for Staphylococcus epidermidis. This method distinguished S. epidermidis from
other staphylococci and specifically quantified the ba