川芎药材极性部分特征性指纹图谱研究.doc

川芎药材极性部分特征性指纹图谱研究.doc川营药材极性部分特征性指纹图谱研究
作者：彭红，付建武，周玉春，黄丽芸
【摘要】目的研究川萼药材极性部分高效液相色谱(HPLC)指纹图 谱测定方法。方法以阿魏酸为对照，采用Eclipse XDB-C18(0DS-2, 250 mmX4. 6 mm, 5 Um)色谱柱，流动相为甲醇T%醋酸梯度洗脱，流速 1. 0 ml/min,柱温25°C ,检测波长298 nm。结果共确定10个共有峰, 其中7号峰为阿魏酸峰，为该指纹图谱的参照峰。供试品溶液的指纹 图谱与对照指纹图谱中的全部10个共有峰对应，且顺序一致。结论实 验方法简单、准确，研究所得的川萼指纹图谱稳定性、重复性好，可 作为川萼极性部分的特征性指纹图谱。
【关键词】川萼；指纹图谱；高效液相色谱
Abstract： ObjectiveTo study the detection methods with fingerprint chromatogram for polar parts of Chuanxiong Rhizome. MethodsWith ferulenol as the reference substance, Eclipse XDB-C18 (ODS-2, 250 mmX 4. 6mm, 5 u m) was used as the chromatographic column. The mobile phase was methanol and 1% HAC, the temprature was 25°C,the flow rate was 1. 0 ml/min, and the wavelength was 238 nm. Results There were 10 peaks , of which the seventh was the ferulenol peak, the reference peak. The ten peaks also existed in the sample just as in the reference solution, whose order was corresponded. ConclusionThe method is simple and accurate with good reproducibility and stability and can be used as the polar characteristic fingerprint atlas.
Key words : Chuanxiong Rhizome: Fingerprint
chromatogram; HPLC
川萼为伞形科植物川萼Ligusticum chuanxiong 燥根茎，用于血淤气滞所致的月经不调、痛经闭经，肝郁气滞而致血 行不畅的胸胁疼痛，风寒湿痹，跌打肿痛,头痛，风湿疼痛[1],近年 来，临床常用于治疗冠心病、心绞痛，缺血性脑血管病等心血管疾病和 妇科疾病。根据国家药品监督管理局在2000年颁发《中药注射液指纹 图谱研究的技术要求（暂行）》（国药管注[2000] 348号）[2],本实验 运用指纹图谱相似度评价软件，对川萼药材进行了指纹图谱研究和比 较研究。现报道如下。
1器材
Agilent 12