骨髓间充质干细胞向纤维软骨细胞表型的诱导分化.pdf

中国组织工程研究与临床康复 第 14 卷 第 2 期 2010–01–08 出版
Journal of Clinical Rehabilitative Tissue Engineering Research January 8, 2010 ,


骨髓间充质干细胞向纤维软骨细胞表型的诱导分化*★
蔡贵泉，崔一民，陈晓东
Differentiation of bone marrow mesenchymal stem cells into fibrochondrocyte phenotype
Cai Gui-quan, Cui Yi-min, Chen Xiao-dong
Abstract
Department of BACKGROUND: The meniscus has limited ability in repairing itself after being injured. However, tissue engineering provides a
Orthopaedics, Xinhua new way to meniscus repair after injury. Bone marrow mesenchymal stem cells (BMSCs), which possess the potential of
Hospital Affiliated to multi-directional differentiations, can be ideal seed cells in meniscus tissue engineering.
Shanghai Jiao Tong
University School of OBJECTIVE: To investigate the feasibility of differentiation of in vitro cultured porcine BMSCs into fibrochondrocyte phenotypes in
Medicine, Shanghai inductive medium.
200092, China METHODS: BMSCs were isolated with whole bone marrow culture method. Then, BMSCs of the third passage were digested and
incubated in a medium containing transforming growth factor-β1, insulin-like growth factor-Ⅰ, dexamethasone and ascorbic acid in
Cai Gu