Estuarine, Coastal and Shelf Science 233 (2020) 106540
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The role of chorion around embryos in toxic effects of bisphenol AF
exposure on embryonic zebrafish (Danio rerio) development
Yang Yang a,b,*, Tian-Le Tang c, Ya-Wen Chen a,b, Wen-Hao Tang a,b, Fei Yang a,b
a Department of Environmental Science and Engineering, Hainan University, Haikou, 570228, PR China
b Haikou Key Laboratory of Environmental Toxicology, Haikou, 570228, PR China
c School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, 571199, PR China
ARTICLE INFO ABSTRACT
Keywords: Bisphenol AF (BPAF) has been found ubiquitously in the environment and attributed to the population degra
Bisphenol AF dation of the fish community. It was proved that the susceptibilities of fish to BPAF exposure are diverse in
Chorion different developing stages. However, the role of embryonic chorion under BPAF exposure remains unknown. In
Dechorionation
the study, model organism zebrafish( Danio rerio) was adopted to carry out the embryo acute toxicity tests (OECD
Microinjection
No. 236) combined with microinjection (MI, at the blastula) and dechorionation (DC, at the pharyngula) tech
Zebrafish
niques and non-treatment (NT-1, NT-2) for investigating the role of chorion around embryos. The results showed
that the BPAF exposure could cons
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