《3,5-二硝基水杨酸比色法测定黄芪多糖的含量》.pdf.pdf

115物研究中国民族民间医药 The medicine study Chinese Journal Ethnomedicine andEthnopharnmcy ·13· 3,5一二硝基水杨酸比色法测定黄芪多糖的含量夏春森1 韦史利2钟艳红1 (,广东,广州,510663; ,新药开发与研究中心,广东,广州,510405) 目的:建立黄芪有效部位多糖的含量测定方法。方法:用优化后的3,5一二硝基水杨酸(DNS)比色法测定黄芪有效部位多糖的台量。结果:"C,显色时间为5rain,%.Rsn=%(n=5)。结论:,重复性好,可用于测定黄芪多糖的含量。【关键词】黄芪;多糖;3,5一二硝基水杨酸法【中图分类号】 【文献标识码】A 【文章编号】1007—8517(2009)08—0013—02 Determination harides inAstragalus membranaceus with DNS Method XlA Chun—sen91 wEI sk一蹬 zHoNG Yan一}∞n一( River Pharmaceutical Holding Guangzhou Hairui Pharmaceutical CO.-lad-Guangzhou ; Drug Research and Development Center-Guangzhou Univers蚵of Traditional Chinese Medicine-Guangzhou ) Objective ToesudMish Bmethod determination Using op曲ized DNS colodmetricmethod detemaine TI他conditionsw骶柏follows: acid 0hal-aqueous bathtemperaturel00℃。and activetime goodliner betw,∞nthe ha- and absodmme毗the wavelength 500nm.‰average meovery %。BSD=%(n=5).Conclusion Themeth- od with伊od reproducibilityand咖be used thedetermination pdysaceharidesAst阳咖m咖曲岫廿【Keywerdsl pob伯啪118ride;A曲阻galIls m即lbmn∞e岫; colorlmewy 黄芪是豆科植物蒙古黄芪AB仃agal瑚membranaceus (Fisch.)]iC'(Bge.)Hisao或膜莫黄芪A8· t