海马神经元细胞HT22T糖氧剥夺模型的建立.pdf

第9届北京国际康复论坛 The 9 thBeijing International Forum on Rehabilitation 1678 National Institutes of Health Stroke Scale (NIHSS). Flow cytometry analysis was employed to detect the proportion of DCs subtypes in the peripheral blood. ResultsNo obvious difference was founded in DCs between ischemic stroke patients and the controls (P=). The numbers and percentages of peripheral blood myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) were significantly different in two groups (P<). ConclusionPatients with ischemic stroke has decreased levels of circulation mDCs and pDCs. These results vertified the function of inflammation in ischemic stroke. Key words:Ischemic stroke; Dendritic cell; Myeloid dendritic cell; Plasmacytoid dendritic cell 海马神经元细胞HT22T糖氧剥夺模型的建立王文竹 1,赵立希 1,白帆 1,陈惠 1,刘丽旭 2 [摘要]目的:采用糖氧剥夺法建立小鼠海马神经元细胞HT22离体拟缺血模型,为研究脑缺血类疾病及药物治疗提供基础。方法:采用细胞计数法获得HT22细胞生长曲线,从而确定细胞铺板的时间和密度,为建立体外缺血模型建立实验基础。建立缺血模型时,将实验分为正常组与糖氧剥夺组(oxygen-glucose deprivation model, OGD),采用酶标仪进行细胞活性检测。结果:与正常组相比,OGD18h诱导细胞死亡率上升,差异具有统计学意义(P<)。结论:HT22糖氧剥夺模型建立成功。该模型对进一步脑缺血机制的研究和神经保护药类的高通量筛查提供了实验基础。[关键词] 海马细胞;糖氧剥夺模型;细胞活性 Establishment of oxygen-glucose deprivationmodel ofHippocampal neurons HT22 Wang Wenzhu 1,Zhao Lixi 1,Bai Fan 1,Chen Hui 1,Lixu Liu 2 1. Institute of Rehabilitation Medicine of China, China Rehabilitation Res