武汉大学期末考试试题.doc

武汉大学生命科学学院 2003 - 2004 学年第一学期期末考试《分子生物学》试卷及参考答案 Final exam of Molecular Biology Course (Spring 2004) 写在参考答案前面的话: ?该课程考试目的是考查学生对所学知识掌握的情况,除选择题外,其他题目的答案基本都不是唯一的。你可以从不同的角度去阐明一个概念。?公布参考答案的目的:为该课程画个句号,让学生过个安心暑假? Best wishes to allof you PART I: DESCRIPTION (2 points each ) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary. 1. Yeast artificial chromosome: 1酵母人工染色体( point ) 2 ponents required for replication and segregation of the natural yeast chromosome, including two telomeric sequences (TEL), one centromere (CEN) and one autonomously replicating sequences (ARS) ( point ) 3 contains genes act as selective markers in yeast and proper restriction sites ( point ) 4c an modate genomic DNA fragments of more than 1 Mb (0. 5 point) 2. RNA interference: 1 RNA 干扰( point ) 2a conserved biological response to double-stranded RNA (1 point) 3 regulates the expression of protein-coding genes through siRNA or miRNA (1 point) 4 the dsRNA is restricted by DICER, then RISC mediates the siRNA and miRNA related RNA degradation and translation inhibition, respectively. (1 point) 3. Proteomics 1蛋白组学( point ) The study of the proteome using techniques of high resolution protein separation and identification ( point) 4 The best separation method is two dimensional gel electrophoresis, the individual protein spots are then cut from the gel and treated with protease to produce a set of peptides characteristic of that protein. The precise masses of each peptide in the sample are then determined by MALDI mass spectrometry. The resulted peptide mass fingerprint of that protein is pared toa database to deduce the function of that protein etc. ( point) 4. Shine-Dalgarno sequence 1 SD 序列( point ) 2A conserved sequence 8-13 nt upstream of the first codon tobe translated (1 point). 3 This sequence was discovered by Shine and Dalgarno ( point) 4 The sequ