培养条件对大肠杆菌的影响-word资料(精).doc

山东职业学院毕业论文题目: 浅析培养条件对大肠杆菌的生长和 rhG-CSF 表达的影响系部: 生物工程系专业: 生物技术及应用班级: 生物技术及应用 0931 班姓名: 学号: 指导教师: 2012 年 03月 30 山东职业学院山东职业学院毕业设计(论文)任务书班级学生姓名指导教师设计(论文)题目浅析培养条件对大肠杆菌的生长和和 rhG-CSF 表达的影响主要研究内容培养条件对大肠杆菌的生长和 rhG-CSF 表达的影响。主要从温度, PH 值, 溶解氧,接种量,培养时间,摇床的转速,工程菌生长时间机诱导时间的对照实验,来研究大肠杆菌的生长和表达的条件。根据研究的结论,进一步分析大肠杆菌在生物药品生产中的应用最佳条件。主要技术指标或研究目标借鉴了大量的著名专家的文献,以文献的知识为主线。严格每一步的操作。逐一研究大肠杆菌的生活条件,对照实验结果,得出更严谨的数据。通过试验得到利于大肠杆菌生长的营养环境和 rhG-CSF 表达的最佳环境条件, 用来提高大肠杆菌工程菌生长和目标蛋白表达,进一步的缩短生产周期,降低生产成本, 为大肠杆菌表达的目的蛋白大规模生产奠定最坚实的基础。基本要求严格根据实验步骤合理安排实验, 每个实验都设立3 个对照, 使实验结果更具有说服力,真实可行。主要参考资料及文献[1] 杨炜, 王伟刚, 田海英,等. 重组大肠杆菌高表达高密度发酵研究[J] . 生物技术 2006 [2] 郑大勇, 罗荣城. 蔡红兵. 基因工程抗体 anti?HBsAgFab 原核表达体系大规模培养条件的实验研究[J] .第一军医大学学报, 2004 [3] 霍向东, 石玉瑚. 大肠杆菌高密度发酵补料调控策略的研究进展[J]. 新疆农业科学。 2004 [4] 张嗣良. 发酵过程多水平问题及其生物反应器装置技术研究, 基于过程参数相关的发酵过程优化与放大技术[J] .中国工程科学. 2001 山东职业学院目录 1前言································································································· -1- 简述································································································ -1- 2材料与方法···················································································· -2- 菌种································································································ -2- 试剂································································································ -2- 培养基及组成成分添加········································································ -2- 培养方法·························································································· -2- 摇瓶发酵······················································································· -3- 种子罐培养···················································································· -3- 3培养条件对工程菌的生长和rhG-CSF 表达的影响················ -3- 培养温度·························································································· -3- 值································································································ -3- 溶解氧··