海洋酵母对樱桃番茄采后链格孢病害防治效果研究.pdf

Biocontrol of Alternaria alternata on Cherry Tomato Fruit by
Use of marine yeast Rhodosporidium paludigenum Fell &
Tallman1
Wang Yifei a，Feng Wu b，Shen Danhong a，Yu Ting a，Zheng Xiaodong a,*
a Department of Food Science and Nutrition，Zhejiang University，Hangzhou（310029）
b College of Food Science and Technology，Huazhong Agricultrual University，Wuhan（430070）

Abstract
The vasidiomycetous yeast Rhodosporidium paludigenum Fell & Tallman, which was isolated from the
south of East China Sea was evaluated for its activity in reducing postharvest decay of cherry tomatoes
caused by Alternaria alternata in vitro and in vivo test. The result showed that washed cell suspensions
of R. paludigenum provided better control of A. alternata than other treatments. Autoclaved cell culture
failed to provide protection against the pathogen. The concentration of antagonist had significant effect
on biocontrol effectiveness. When the washed yeast cell suspension reached the concentration of 1×109
CFU/ml and challenged with pathogen spore suspension at 5×104 spores/ml, the rate of decay was only
37% after 5-day incubation at 25℃ . Efficacy of R. paludigenum was obvious when it was applied
simultaneously or prior to i