乙醇对HepG2细胞VASN蛋白表达与分泌水平的影响研究.pdf

2021 11 27 33 620 Contemporary Medicine Issue
当代医学 年 月otein (VASN) in HepG2 cells stimulated by ethanol and analyze the effect of ethanol on
the expression and secretion of VASN protein in HepG2 cells. Methods HepG2 cells were stimulated with different concentrations of ethanol, and
collected at 24 h, 48 h and 72 h, respectively. The VASN mRNA level of HepG2 cells was detected by quantitative nucleic acid amplification detec-
tion system (qPCR). The secretory level of samples after ultrafiltration concentration was detected, and the effect of ethanol on the VASN protein ex-
pression and secretion level of HepG2 cells were analyzed. Results Compared with no ethanol stimulation, the VASN mRNA level of HepG2 cells
stimulated by 25 mmol/L ethanol, 50 mmol/L ethanol and 100 mmol/L ethanol was significantly higher at 24 h, 48 h and 72 h; compared with no eth-
anol stimulation, the VASN protein secretion level of HepG2 cells stimulated by 25 mmol/L ethanol, 50 mmol/L ethanol and 100 mmol/L ethanol was
significantly higher at 24 h, 48 h and 72 h. Conclusion Ethanol can promote the expression and secretion of VASN in HepG2 cells, and provide an
effective reference for biological cell research.
Key words: HepG2 cells; Vasn protein expression; Vasn protein secretion; Ethanol
肝癌作为临床常见恶性肿瘤，其主要诱发因素包括脂肪