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widespread occurrence of calicin, a basic cytoskeletal protein of sperm cells, in diverse mammalian species开题资料.pdf


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Differentiation
Differentiation (1988) 38:21-27
specific sperm mor- dymides were obtained from adult Wistar rats, mice, ham-
phology. sters, guinea pigs and bulls. Ejaculates containing human
spermatozoa were from outdated reference samples ob-
tained from the andrology unit of the Department of Der-
matology, Mannheim Medical School, University of Hei-
delberg, FRG. Sperm was recovered from vasa and epididy-
Introduction mides and fractionated into heads and tails as previously
The perinuclear theca is an extranuclear structural element described [17]. After washing with phosphate-buffered sa-
found in mammalian sperm (for reviews, see [4, 10, 17, line (PBS) containing 25 mM EDTA, human sperm cells
261). This structure represents a dense layer of variable from ejaculates were treated in the same manner. Sperm
thickness, which is sandwiched - in the apical portion be- heads were extracted with 2 A4 NaCI, 5 mM dithiothreitol
tween the outer nuclear membrane and the inner acr

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