最新乙型肝炎孪生子和非孪生子HBV preS1S2区准种特性及其与临床表型关系的初步研究.docx


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乙型肝炎孪生子和非孪生子HBV preS1S2区准种特性及其与临床表型关系的初步研究
第三军医大学硕士学位论文
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A
Variation,enviroment and clinical manifestation.
In this paper,the twins and sibling pairs infected with HBV as objectives,we preliminary investigated the relationship between the quasispecies of preS 1/$2 region of HBV and the clinical manifestation of twins and siblings.Zygosity of twins was diagnosed
by microsatellite polymorphism.To identify the serological model and exclude the
evidence of coinfection with other virus,we detected HAV~HEV serological markers by ELISA method.HBV DNA level was detected by Lightcycler Fluorescent Quantitative system and Liver function(ALT,AST,TBil)was detected by HITACHl7250 Biochemistry detection system.We want to clarify the role of host factors to affect the clinical phenotype of hepatitis B through comparatively analyzing the model of serological markers,infectious rate,concordance rate,diseases phenotype concordance rate,the patterns of serological
markers among the twins and siblings.We obtained 6 patients with high HBV DNA level
and were divided into 3 groups.The experimental procedure was LPCR(amplification of 3.2kb genome DNA of HBVl一TA cloning-quasispecies detection-nucleotide sequence analysis.
The major research results are as follows:
I.Among 20 pairs of twins infected with HBV or with high risk to infection,the zygosity of 14 pairs of MZ twins and 6 pairs of DZ twins were identified by Genescan with 28 STR markers,the probability that any twins pair were MZ if all markers were concordant was 99.99999999%.
2.The genotype B and C of HBV were found in these 6 patients.Specifically,one pair of MZ twins were infected by HBV genotype B and C respectively which indicated the source of pathogen was different,one pair of DZ twins were infected by HBV genotype B
and one sibling pair were infected by HBV genotype C.
3.The completion of full-length HBV LPCR and cloning will be a basis fo

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  • 时间2022-01-26