山羊卵母细胞成熟期间葡萄糖代谢作用对卵母细胞胞质成熟的影响.pdf

山羊卵母细胞成熟期间葡萄糖代谢作用对卵母细胞胞质成熟的影响2 5)向成熟培养液中单独添加糖酵解途径抑制剂碘醋酸酯(iodoacetate)卵母细胞内GSH/GSSG与GSX均显著下降。综上所述,在成熟培养液中分别添加PPP和糖酵解途径抑制剂均能够降低卵母细胞与胚胎发育能力。其中成熟期间糖酵解途径被阻断后对胚胎发育能力的影响更加明显,卵母细胞成熟期间糖酵解途径被抑制后大部分胚胎卵裂率下降。PPP途径与糖酵解途径被阻断后显著降低卵母细胞内GSX与GSH含量,从而影响胚胎发育能力。本文成功的利用该实验系统通过胚胎发育与GSX等指标首次研究了山羊卵母细胞成熟期间葡萄糖代谢途径被阻断后对胞质成熟以及胚胎发育能力的影响。为卵母细胞成熟期间葡萄糖代谢对胚胎发育能力的影响奠定了理论基础,并对临床治疗糖尿病的治疗具有理论指导意义。关键词:山羊;卵母细胞;体外成熟;糖酵解;PPP;胞质成熟;谷胱甘肽;胚胎发育山东农业大学硕士学位论文3 Effects of etabolism during maturation on the cytoplasm of goat oocytes ABSTRACT We know that the metabolism of oocytes during maturation is very important for the resumption of oocytes and the cytoplasmic the development of modern society, people's living standards, and the increased social pressure more and more people are suffering from diabetes and obesity the causes of diabetes and obesity so that changes in blood sugar and thus affect the reproductive system. Many people suffering from diabetes or obesity caused changes in glucose concentration within the follicle and the changes of oocytes glucose metabolism, resulting in abnormal maturation of nuclear and cytoplasm which affects fertility. Therefore, there is an important theoretical guidance and clinical significance to study the relationship between metabolism of oocytes and maturation of nuclear and cytoplasm. Glucose is the main sources for metabolism in oocytes and it is an important role during the maturation of nuclear and cytoplasm. There are four main metabolic pathways with glucose including glycolysis, the pentose phosphate pathway (PPP), the hexosamine biosynthesis pathway (HBP) and the polyol pathway. Of which glycolysis and pentose phosphate pathway is more prominent role. Although many researchers studied the effects of the glucose in follicular on oocytes and the effects of supplementation of glucose metabolism inhibitor on the GSH in the oocytes, for the in vitro maturation of oocytes, especially in the chemical defined medium system to determine the me