差异超速离心法对不同种属间...细胞外泌体分离的可靠性研究 赵仁礼.pdf

中国临床解剖学杂志
Chinese Journal of Clinical Anatomy
ISSN 1001-165X,CN 44-1153/R
镜、纳米颗
粒示踪分析及 Western Blot 鉴定分离纯化的外泌体。 结果 通过对成骨、成脂、成软骨分化后染色，大鼠
及小鼠 BMSCs 具备干细胞特有的多向分化潜能。流式检测结果显示大鼠和小鼠 BMSCs 表达干细胞标
志性表面抗原；透射电镜下，小鼠及大鼠 BMSCs 外泌体呈典型双层膜的杯托结构；平均粒径大小为 107
nm 和 152 nm；Western Blot 结果显示其表达外泌体标志性蛋白。 结论 我们能成功分离培养大鼠及小
鼠的骨髓间充质干细胞；同时，差异超速离心法能够可靠地分离纯化出大鼠及小鼠骨髓间充质干细胞来
源的外泌体。
【关键词】骨髓间充质干细胞； 外泌体； 差异超速离心法
【中图分类号】Q249 【文献标识码】A
Study on the reliability of differential hypervelocity centrifugation for isolation of mesenchymal stem
cells derived exosomes from different species
Zhao Renli1,2, Lai Guohua1, Wu Jiachang1, Wu Mingjie1, Zhuang Weida1, Ouyang Jun3*, Sang Hongxun1,2*
1. Department of Orthopedics, Shenzhen Hospital of Southern Medical University, Shenzhen 518100, China;
2. The Third Clinical Medical College of Southern Medical University, Guangzhou 510630, China; 3.
Department of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Southern
Medical University, Guangzhou 510515, China
【Abstract】 Objective To isolate and culture the mouse and