抗TMV短N导入片段烤烟近等基因系降低了抗性连锁累赘 刘勇.pdf

: .
分子植物育种)。本研究以短 N 导入片段烤烟近等
基因系为材料，设置正常施氮量和 20%高施氮量 2 个施肥量处理，测定成熟期上部叶叶绿素含量和叶片厚
度、测产和常规化学成分测定，分析短 N 导入片段材料连锁累赘是否改善。结果表明，与‘Y87NL’ (长 N
导入片段)相比，‘Y87NS’ (短 N 导入片段)采烤期上部第 3 片叶叶绿素含量接近对照‘云烟 87’。‘Y87NS’可
有效减少‘Y87NL’打顶后上部叶叶绿素含量偏高的连锁累赘。在无 TMV 病害的情况下，‘Y87NS’改善了
‘Y87NL’产值、均价和上等烟比例偏低、中部和上部叶的烟碱和总氮含量偏高的缺点。‘Y87NS’高抗 TMV、
降低了长 N 导入片段在烤烟‘云烟 87’中的连锁累赘，具有良好的育种价值。
关键词 烤烟; 短 N 导入片段; 叶绿素; 叶片厚度; 产值; 烟碱
Short N Introgression Fragments Near-isogenic Lines Reduced the
TMV Resistance Linkage Drag in Flue-cured Tobacco
Liu Yong * Yu Haiqin Yuan Cheng Zeng Jianmin Huang Changjun *
Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center,
Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021
* Co-corresponding authors, ******@; ******@

基金项目：本研究由中国烟草总公司云南省公司科技项目(2018530000241003)资助
引用格式：Liu Y., Yu ., Yuan C., Zeng ., and Huang ., 20XX, Short N Introgression fragments near-isogenic lines reduced the
TMV resistance linkage drag in flue-cured tobacco, Fenzi Zhiwu Yuzhong (Molecular Plant Breeding), 0(0): 0000-0000 (刘勇, 于海芹,
袁诚, 曾建敏, 黄昌军, 20XX, 抗 TMV 短 N 导入片段烤烟近等基因系降低了抗性连锁累赘, 分子植物育种, 0(0): 0000-0000)分子植物育种
Molecular Plant Breeding
Abstract Long introgression fragments of the N gene derived from Nicotiana glutinosa in the flue-cured
tobacco confer Tobacco mosaic virus (TMV) resistance, with adverse traits (linkage drag). In this paper, the near
isogenic lines of short N introgression