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年 月 四川大学学报 自然科学版
２０２２ ３ ( ) 酵母双杂交实验 双分子
: １１;ABA ４; ; ;
荧光互补实验
中图分类号 文献标识码
:Q９４５ :A DOI:１０．１９９０７/j．０４９０Ｇ６７５６．２０２２．０２６００１
ResearchontheinteractionbetweenCPK andABF
１１ ４
HUBo LIUYinＧYin YANGYi
, g g,
(KeyLaboratoryofBioＧResourceandEcoＧEnvironmentofMinistryofEduction,
CollegeofLifeSciences,SichuanUniversity,Chengdu６１００６５,China)
Abstract
:ToinvestigatethewaythatthecalciumＧdependentproteinkinaseCPK１１involvedinregulating
ABAsignaltransduction,Yeasttwohybrid(Y２H)assayandBimolecularfluorescencecomplementation
(BiFC)assaywereusedtoanalyzetherelationshipbetweenCPK１１andABAＧresponsisveelementbindＧ
ingfactorsABF４．Y２HassayshowedthatCPK１１andABF４hadaninteractioninvitro,andBIFCassay
invivo
showedthatCPK１１andABF４hadaninteraction ．Theaboveexperimentstogetherprovedthat
therewasadirectinteractionbetweenCPK１１andABF４．Asahomologousprotein
２０２２ ３ ( ) 酵母双杂交实验 双分子
: １１;ABA ４; ; ;
荧光互补实验
中图分类号 文献标识码
:Q９４５ :A DOI:１０．１９９０７/j．０４９０Ｇ６７５６．２０２２．０２６００１
ResearchontheinteractionbetweenCPK andABF
１１ ４
HUBo LIUYinＧYin YANGYi
, g g,
(KeyLaboratoryofBioＧResourceandEcoＧEnvironmentofMinistryofEduction,
CollegeofLifeSciences,SichuanUniversity,Chengdu６１００６５,China)
Abstract
:ToinvestigatethewaythatthecalciumＧdependentproteinkinaseCPK１１involvedinregulating
ABAsignaltransduction,Yeasttwohybrid(Y２H)assayandBimolecularfluorescencecomplementation
(BiFC)assaywereusedtoanalyzetherelationshipbetweenCPK１１andABAＧresponsisveelementbindＧ
ingfactorsABF４．Y２HassayshowedthatCPK１１andABF４hadaninteractioninvitro,andBIFCassay
invivo
showedthatCPK１１andABF４hadaninteraction ．Theaboveexperimentstogetherprovedthat
therewasadirectinteractionbetweenCPK１１andABF４．Asahomologousprotein
拟南芥钙依赖蛋白激酶CPK11与ABA响应元件结合因子ABF4相互作用的研究 来自淘豆网www.taodocs.com转载请标明出处.
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