兰燕月 张饮江 宋增福 吴圣哲 徐成龙 赵志淼 摘要:【目的】设计一种可有效降低海水暂养循环系统中氮浓度的新型脱氮技术工艺,提 was used as carbon source and biofilm carrier to establish simultaneous nitrification and denitrification system by domesticating low-temperature denitrifying bacteria(nitrifying bacteria and denitrifying bacteria) combined with artificial enhanced film-hanging. 【Result】Proteobacteria and Bacteroidetes were the main components of nitrifying bacteria and denitrifying bacteria enrichment solution after acclimation at low temperature and high salinity, but gammaproteobacteria and alphaproteobacteria were the main components of nitrifying bacteria enrichment solution at class level, and their relative abundances were % and % respectively. The relative abundance of gammaproteobacteria in denitrifying bacteria enrichment solution was %. By scanning electron microscopy, it was found that microbial membrane covered the surface of corn cob in denitrification reactor, and the number of pore in the surface decreased greatly. Corn cob was also used as so-lid carbon source to promote denitrification. Within 60 d of operation of corn cob denitrification reactor, the removal rates of total nitrogen, ammonia nitrogen and nitrate nitrogen in outlet water increased first and then decreased with time. The highest removal rates were(±)%, (±)% and (±)% respectively. 【Conclusion】Corn cob denitrification reactor with corn cob as carbon source and biofilm carrier and artificial strengthening film formation can realize simultaneous nitrification and denitrification process. The effect of denitrification is good and the system can run for a long time. It also has the characteristics of simple construction process, small size and low cost. It is suitable for most marine products temporary maintenance system. Key words: seawater temporary nutrition circulation system; low temperature; corn cob; simultaneous nitrification and denit