吡喃酮对黑土微生物群落结构的影响 司马鑫琪.pdf

2022 50 5 690-697
山西农业科学 ， （ ）： Journal of Shanxi Agri9，China；
of Forestry，Nanjing Forestry University，Nanjing 210037，China；
of Agriculture，Shanxi Agricultural University，Taigu 030801，China）
Abstract：Pyrone, a derivative of allelochemicals, has significant inhibitory effect on the growth of weeds, and high
security for crops. In order to define its effects on soil microbial community structure, in this study, two additive levels of pyrone
were set including low level( mg/kg, normal application) and high level( mg/kg , simulation of pollution of high mass
fraction), and soil that did not receive any pyrone was set as controls(CK). With high-throughput sequencing technology,
sequencing analysis was conducted on the V3+V4 region of 16S rRNA of soil bacteria and the ITS1 region of fungal treated by
Pyrone for 7 d and 60 d. The results showed that both of the Shannon and Chao1 indexes of bacteria and fungi in the black soil
after 7 d and 60 d of treatment had no significant change compared with CK. Low mass fraction of pyrone significantly increased
the relative abundance of the bacterium Sphingomonas and the fungus Staphylococcus, and significantly reduced the relative
abundance of the fungal Ascomycota after 7 d of incubation