菠萝类纤维素合成酶CslD...AcoCslD2a功能分析 刘朝阳.pdf

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园艺学报
3 个亚组，其
中第 3 亚组成员的数在菠萝中有明显扩增；结合共线性及表达模式分析等预测了家族成员可能的生物学
功能。克隆了 AcoCslD2a 基因 cDNA 全长序列，亚细胞定位分析表明，其编码蛋白定位于高尔基体。构
建 ProAtUbq10 和 ProAtCslD3 启动子驱动的 AcoCslD2a 的植物表达载体，经根癌农杆菌 GV3101 介导转
化至拟南芥 atcsld3 根毛缺失突变体，不同载体的转基因结果一致表明，AcoCslD2a 能够完全恢复突变体
根毛数量至野生型水平，可部分恢复突变体的根毛长度。
关键词：菠萝；CslD；细胞壁；根毛


Identification of CslD Gene Family in Pineapple and Functional Analysis of
AcoCslD2a
LIU Chaoyang*，LIAO Zhichan，LU Xinxin，and HE Yehua
（Colleage of Horticulture，South China Agricultural University/Key Laboratory of Biology and Germplasm Enhancement
of Horticultural Crops in South China，Ministry of Agriculture and Rural Affairs，Guangzhou，Guangdong，510642，China）
Abstract：In this study，eight CslD genes were characterized in pineapple genome. Chromosomal
mapping showed that they were distributed on 7 chromosomes and 1 scaffold. Three to five exons were
identified in these 8 genes. Three subgroups were divided according to the phylogenetic analysis，and the
number of Group 3 members was significantly increased in pineapple. The potential biological functions of
the pineapple CslD gene family members were further predicted，basing on the collinearity and gene
expression analysis. The full length cDNA sequence of AcoCslD2a was cloned and the subcellular
localization analysis indicated that its encoding protein was localized in Golgi apparatus. Two expression
vector for AcoCslD2a gene，which were driven by the promoters including ProAtUbq10 and ProAtCslD3，
respectively，were constructed and transformed into the Arab