多管发酵法测定水中大肠菌群.doc

综合实验报告书( 201 4 ~201 5 学年) 实验题目多管发酵法总测定水中大肠菌群学 院名称生物与食品工程学院专业(班级) 2012 级生物工程姓名(学号) 孙大林 2012215103 201 5年 01月 13日多管发酵法测定水中大肠菌群摘要: 利用多管发酵法检测自来水和池塘水中大肠菌群的数量, 以对不同水样的大肠菌群污染情况做出初步判断。根据? 中华人民共和国国家标准生活饮用水标准检验法? 中有关大肠菌群的检测方法与指标对上述水样进行检测。通过记录阳性管数并查最大可能数表( MPN ) 查出不同水样中大肠菌群的可能含量。多管发酵法的原理是根据大肠菌群细菌能发酵乳糖产酸产气以及具备革兰氏染色阴性,无芽孢,呈杆状等有关特性,通过初(步)发酵试验、平板分离和复发酵试验等三个步骤进行实验, 得出水样中总大肠菌群数, 实验结果以最大可能数 MPN 表示。关键词:多管发酵法、大肠菌群、最大可能数( MPN ) Abstract: the use of multiple tube fermentation test of tap water and the number of coliform group, pond water in different water samples of coliform bacteria pollution make a preliminary judgment. According to "the People's Republic of China national drinking water standard inspection ? in the fecal coliform detection method and index of the water samples for bya positive record number (MPN) maximum possible log tables and check of coliform bacteria may content in different water -tube fermentation principle is based on fecal coliform bacteria can ferment lactose produce acid gas and possess a gram negative, no spore, assumes the rods and so on characteristics, through the early (step) fermentation test, plate separation and plex fermentation test three steps of the experiment, it is concluded that the total number of coliform bacteria in water samples, the experimental results with the greatest possible number of MPN said . Key words: multi-tube f