益气除痰方调控内质网应激反...肺癌细胞上皮间质转化的影响 孙玲玲.pdf

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辽宁中医杂志响。采用 Western blot 方法和定量 PCR 方法检测 A549、H1299、H1650 肺癌细胞上皮间质转化
标志物和内质网应激标志物的表达。结果：益气除痰方可以抑制 A549 细胞的迁移侵袭能力，抑制
A549、H1650、H1299 肺癌细胞的上皮间质转化。TGF-β诱导 A549、H1299、H1650 肺癌细胞内质网
应激标志物 GRP94、XBP-1s、ATF4 的表达，内质网应激抑制剂可以抑制 A549 的上皮间质转化，益
气除痰方对内质网应激反应的 GRP94、XBP-1s、ATF4 等有作用。结论：益气除痰方可能通过调控内
质网应激反应来抑制肺癌细胞上皮间质转化。
关键词：肺癌 上皮间质转化 内质网应激 中药复方 转移

Study on the effect of Yiqi Chutan Recipe on the stress response of
endoplasmic reticulum to inhibit the epithelial mesenchymal transformation of lung
cancer cells*
Sun Lingling1**, Du Haiyan2, Zhang Zhiquan1, He Canfeng2, Tan Yuhui2, Lin Lizhu1***
(1. The First Affiliated Hospital of Guangzhou University of Chinese medicine, Guangzhou 510405, China; 2.
Guangzhou University of Chinese Medicine, Guangzhou 510405, China)
Abstract: Objective To investigate the effect of Yiqi Chutan Recipe on endoplasmic reticulum stress related
genes and epithelial-mesenchymal transitions in lung cancer cell lines A549, H1299 and H1650. Methods TGF - β was
used to induce the epithelial-mesenchymal transitions of A549, H1299 and H1650. Yiqi Chutan Recipe and 4-PBA,
the endoplasmic reticulum stress inhibitor were used to intervene. The effect of Yiqi Chutan Recipe on cell migration
was detected by scratch test. Western blot and quantitative PCR were used to detect the expression of mar