细胞周期蛋白D1和细胞周期蛋白依赖激酶4在宫颈鳞癌中的表达.doc

1 细胞周期蛋白 D1 和细胞周期蛋白依赖激酶 4 在宫颈鳞癌中的表达作者:姬宏宇成日青薄爱华舒丽莎【摘要】目的: 探讨 Cyclin D1 和 CDK4 在正常宫颈上皮、 CIN 和宫颈鳞癌中表达状况。方法: 收集子宫颈活检及手术标本 118 例,其中正常鳞状上皮 14 例, CIN25 例, 宫颈鳞癌79例。采用SP 免疫组织化学方法,检测 CyclinD 1和 CDK 4 蛋白的表达情况。结果: Cyclin D1 和 CDK4 在正常宫颈上皮、 CIN 和宫颈鳞癌的中表达水平存在显著性差异( P005 ), 在不同分化程度的宫颈鳞癌间无显著性差异( P005 )。结论: 在宫颈鳞癌发生过程中伴有 Cyclin D1和 CDK4 表达水平的增高,检测其含量改变有助于宫颈鳞癌诊断。【关键词】宫颈鳞癌,细胞周期蛋白 D1 ;细胞周期蛋白依赖激酶 4 1MATERIALS AND METHODS 11Samples collection 2 Specimens were taken from uterine cervix of 118 cases The surgical material was from hysterectomy or biopsy All samples were confirmed by pathological diagnosis There were normal squamous epithelium in 14 cases and CIN in 25 cases Normal cervices from hysterectomy specimens were removed for lEiomyomas served as controls 79 cases were cervical squamous cell carcinoma, among which 8 cases were well differentiated squamous cell carcinoma, 58 cases were moderately differentiated squamous carcinoma and poorly differentiated squamous cell carcinoma were 13 cases The above mentioned specimens were fixed in 10% formalin, embedded in paraffin and sliced into the thickness of5μm The cellular protein expressions of CyclinD1 and CDK4 were studied with streptavidin peroxidase immunohistochemical method which has high sensitivity and strong specificity 12Immunohistochemistry reagents 2006 年2月 Ji Hongyu,et al:Expression of CyclinD1 and CDK4 Protein in Cervical Squamous Cell