好看的模板.ppt

Analysis of High-throughput Gene Expression Profiling
Why to Measure Gene Expression
1. Determines which genes are induced/repressed in
response to a developmental phase or to an
environmental change.
2. Sets of genes whose expression rises and falls
under the same condition are likely to have a
related function.
3. Features such as mon regulatory motif can be
detected within co-expressed genes.
4. A pattern of gene expression may be used as an
indicator of abnormal cellular regulation.
• A useful tool for cancer diagnosis
Why to Measure Gene Expression in Large Scale?
Transitional vs. High-throughput Approaches
Techniques Used to Detect Gene Expression Level
Microarray (single or dual channel)
SAGE
EST/cDNA library
Northern Blots
Subtractive hybridisation
Differential hybridisation
Representational difference analysis (RDA)
DNA/RNA Fingerprinting (RAP-PCR)
Differential Display (DD-PCR)
aCGH: array CGH (DNA level)
High-throughput
Basic Information of Microarray, SAGE and cDNA Library
(DNA) Microarray
1. Developed around 1987.
2. Employ methods previously exploited in immunoassay context – specific binding and marking techniques.
3. Two types of probes:
Format I: probe cDNA (500~5,000 bases long) is immobilized to a solid surface such as glass; widely considered as developed at Stanford University; Traditionally called DNA microarrays.
Format II: an array of oligon