Tannins of Cornaceous Plants.Ⅱ.cornusiins D,E and F,New Dimeric and Trimeric Hydrolyzable Tannins from Cornus officinalTannins of Cornaceous Plants.Ⅱ.cornusiins D,E and F,New Dimeric and Trimeric Hydrolyzable Tannins from Cornus officinalis
(10)2665-2669(1989)
AnLi
Introduction
In a previous paper ,we reported the isolation of eleven tannins from fruits of Cornus officinalis. Including a dimeric hydrolyzable tannin cornusiinA,and a trimeric hydrolyzable tannin ,cornusiinC.
now we have improved separation method and isolated five additional oligomeric hydrolyzable tannins,among which three are new tannins.
method
The fruits(1250g) of Cornus officinalis were homogenized in 70% acetone ().then was concentrated to 500ml.
the aqueous liquor was subjected to column chromatography over Dia-ion HP-20,the eluate with MeOH in % eluate was subjected column chromatography over Toyopearl HW-40 with 70%EtOH-70% acetone(10:0-9:1-8:2) as eluant . 60% MeOH eluant was subjected column chromatography over Toyopearl HW-40 with 70%EtOH-70 % acetone (10:0-9:1) as eluant.
method
fraction A was separated by column chromatography on MCI-gel CHP-20P with 30% MeOH and then with 40% MeOH.
the aqueous mother liquor was subjected to colum chromatography over Dia-ion HP-20 ,Fractions obtained were further separated by column chromatography on Toyopearl HW-40 and
鞣质的分离检测 来自淘豆网www.taodocs.com转载请标明出处.