心肌缺血预处理对未成熟心肌细胞凋亡的影响.doc

心肌缺血预处理对未成熟心肌细胞凋亡的影响.doc心肌缺血预处理对未成熟心肌细胞凋亡的影响
作者:孙忠东,池一凡,杨铁南,侯文明,牛兆倬,唐子龙【关键词】缺血预处理
摘要:目的探讨心肌缺血预处理(MIP)对未成熟心肌bcl-2、bax、fas蛋白表达的影响。方法采用兔Langendorff模型,24只幼兔随机分为3组,对照组(C,n=8):仅灌注KH液180min,然后取标本;缺血/再灌注组(I/R, n=8):灌注20min后,停灌45min,复灌120min;心肌缺血预处理组(MIP, n=8),灌注20min,反复2次停灌5min,复灌5min,然后重复I/R组缺血/再灌方法。以凋亡细胞原位标记、琼脂糖凝胶电泳检测DN***段梯及bcl-2、bax、fas蛋白表达作为检查指标。结果琼脂糖凝胶电泳检测DN***段梯:MIP组与I/R组比较,光密度明显减弱; MIP组与I/R组比较,bcl-2表达明显增多,bax、fas表达明显减少。结论 MIP通过影响bcl-2、bax、fas基因蛋白的表达减少心肌细胞凋亡。
关键词: 缺血预处理;心肌;细胞凋亡;基因蛋白
The Effects of Myocardial Ischemic Preconditioning on Immature Myocardial Cell Apoptosis
Abstract: OBJECTIVE To investigate the effects of myocardial ischemic preconditioning (MIP) on immature myocardial cell apoptosis and expression of bcl-2,bax,fas protEin .METHODS Isolated odel inutes. Ischemia/reperfusion(I/R) group inutes ,and then kept under ischemia 45 minutes, folloinutes. Myocardial ischemic preconditioning (MIP) group inutes ,and then repeatly ischemia 5 minutes folloinutes 2 times,finnaly reperfused 120 minutes. Such indexes, including the TUNEL, nucleosomal ladder of DNA fragments, and the expression of bcl-2, bax,and fas gene ents,expression of bax and fas gene,and increase in expression of bcl-2 This study demonstrated that MIP decrease immature myocardial cell apoptosis by affecting the expression of bcl-2, bax,and fas gene .
Key ic preconditioning;Myocardial; Apoptosis;Gene protein