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HUAZHONG AGRICULTURAL UNIVERSITY
烟草叶片愈伤组织诱导与植株再生的研究
Research on Callus Induction and Plant
Regeneration of o Leaves
姓名:
黄金波
CANDIDATE:
Huang-Jin Bo
学号:
20**********
STUDENT ID:
20**********
课程:
植物细胞工程实验
CURRICULUM:
Plant Cell Engineering Experiment
班级:
MAJOR:
生技1201班
Biotechnology 1201
指导老师:
SUPERVISOR:
柳俊齐迎春陈浩
Liu-Jun Qi-Ying Chun Chen-Hao
中国 武汉
WUHAN,CHINA
二○一四年十二月
DEC, 2014
烟草叶片愈伤组织诱导与植株再生的研究
黄金波
华中农业大学生命科学技术学院生技1201班
[摘要] [目的]以烟草叶片为材料,通过这次实验,基本掌握植物愈伤组织诱导与植株再生的原理和方法,熟练掌握相关实验操作技术;另外,通过本次实验,完成烟草植株再生,并对比光照和黑暗等不同方法,对诱导愈伤组织状态和诱导率的影响,进行结果分析。[方法]在无菌条件下,把烟草叶片剪成1cm2大小的小方块, BA的MS诱导培养基在光照和黑暗条件下诱导培养;三周后, BA的MS分化培养基中光照培养;两个月后,观察结果,统计分析。[结果]诱导培养后,%的被污染了;%全部接种到分化培养基上培养。结果表明分化率在光照条件下和在黑暗条件下无差别;每个愈伤组织分化芽数在光照条件下明显多于在黑暗条件下诱导;分化芽状态在黑暗和光照条件有一定的区别,分化植株苗在光照和黑暗条件下诱导没有明显区别。[结论]光照条件和在黑暗条件下诱导可以影响愈伤分化芽数和分化芽状态。
[关键词] 烟草叶片;愈伤组织;细胞工程;分化;诱导;植株再生
Research on Callus Induction and Plant
Regeneration of o Leaves
Huang-Jin Bo
Huazhong Agriculture University , College of Life Science and Technology , Biotechnology 1201
[Abstract] [Objective]Using o leaves as materials, through the experiment, we should handle the basic principle and method of plant callus induction and plant regeneration,master related experiments’ technology; In addition, through this experiment, plete the o plant regeneration, pared different methods of callus induction, such as light and dark state, and the effects to the state and induction rate, then analyzing the results. [Method]The o leaf was cut into 1 cm2 size small squares under aseptic conditions. Firstly induced in the MS induction medium with mg/L NAA and mg/L BA, under the condition of light and dark, respectively; Three weeks later, transferred to MS differentiation medium with mg/L NAA and mg/L BA culturing under light; Two months later , observing the results, and analyzing the statistics. [Results]After the induction culturing
烟草叶片愈伤组织诱导与植株再生的研究
Research on Callus Induction and Plant
Regeneration of o Leaves
姓名:
黄金波
CANDIDATE:
Huang-Jin Bo
学号:
20**********
STUDENT ID:
20**********
课程:
植物细胞工程实验
CURRICULUM:
Plant Cell Engineering Experiment
班级:
MAJOR:
生技1201班
Biotechnology 1201
指导老师:
SUPERVISOR:
柳俊齐迎春陈浩
Liu-Jun Qi-Ying Chun Chen-Hao
中国 武汉
WUHAN,CHINA
二○一四年十二月
DEC, 2014
烟草叶片愈伤组织诱导与植株再生的研究
黄金波
华中农业大学生命科学技术学院生技1201班
[摘要] [目的]以烟草叶片为材料,通过这次实验,基本掌握植物愈伤组织诱导与植株再生的原理和方法,熟练掌握相关实验操作技术;另外,通过本次实验,完成烟草植株再生,并对比光照和黑暗等不同方法,对诱导愈伤组织状态和诱导率的影响,进行结果分析。[方法]在无菌条件下,把烟草叶片剪成1cm2大小的小方块, BA的MS诱导培养基在光照和黑暗条件下诱导培养;三周后, BA的MS分化培养基中光照培养;两个月后,观察结果,统计分析。[结果]诱导培养后,%的被污染了;%全部接种到分化培养基上培养。结果表明分化率在光照条件下和在黑暗条件下无差别;每个愈伤组织分化芽数在光照条件下明显多于在黑暗条件下诱导;分化芽状态在黑暗和光照条件有一定的区别,分化植株苗在光照和黑暗条件下诱导没有明显区别。[结论]光照条件和在黑暗条件下诱导可以影响愈伤分化芽数和分化芽状态。
[关键词] 烟草叶片;愈伤组织;细胞工程;分化;诱导;植株再生
Research on Callus Induction and Plant
Regeneration of o Leaves
Huang-Jin Bo
Huazhong Agriculture University , College of Life Science and Technology , Biotechnology 1201
[Abstract] [Objective]Using o leaves as materials, through the experiment, we should handle the basic principle and method of plant callus induction and plant regeneration,master related experiments’ technology; In addition, through this experiment, plete the o plant regeneration, pared different methods of callus induction, such as light and dark state, and the effects to the state and induction rate, then analyzing the results. [Method]The o leaf was cut into 1 cm2 size small squares under aseptic conditions. Firstly induced in the MS induction medium with mg/L NAA and mg/L BA, under the condition of light and dark, respectively; Three weeks later, transferred to MS differentiation medium with mg/L NAA and mg/L BA culturing under light; Two months later , observing the results, and analyzing the statistics. [Results]After the induction culturing
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