Distribution of insulin mRNA transcripts within the human body.pdf.pdf

Biochemical and Biophysical munications 451 (2014) 425–430
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Biochemical and Biophysical munications
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Distribution of insulin mRNA transcripts within the human body
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Glenn D. Bell, Shiva Reddy, Xueying Sun 1, Yi Yang, Geoffrey W. Krissansen
Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, University of Auckland, Auckland 1005, New Zealand
article info abstract
Article history: Here we sought evidence for the existence of insulin mRNA-producing cells outside the human pancreas.
Received 22 July mercially plementary DNA (cDNA) arrays prepared from 72 different types of adult
Available online 7 August 2014 human tissues were screened by PCR for transcripts encoding insulin, and other classic pancreatic hor-
mones. Insulin mRNA transcripts were detected by standard PCR in the pancreas, stomach, pylorus region
Keywords: of the stomach, and the duodenum; and additionally by nested PCR in the jejunum, ileum and cecum, but
Enteroendocrine not in other body tissues including the brain and colon. Most of these tissues also variably expressed
Gastrointestinal tract
mRNA transcripts for amylase a2B, amylin, glucagon, somatostatin, and pancreatic polypeptide. In
Insulin mRNA
summary, using sensitive PCR methods we have provided evidence for the presence of rare i