Induction of miR-29a by saturated fatty acids impairs insulin signaling and glucose uptake through translational repression of IRS-1 in myocytes.pdf.pdf

FEBS Letters 588 (2014) 2170–2176
journal homepage:
Induction of miR-29a by saturated fatty acids impairs insulin signaling
and glucose uptake through translational repression of IRS-1 in
myocytes
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Won-Mo Yang a, Hyo-Jin Jeong a, Seung-Yoon Park a, Wan Lee a,b,
a Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea
b Endocrine Channelopathy, Channelopathy Research Center, Dongguk University College of Medicine, Goyang 410-773, Republic of Korea
article info abstract
Article history: MicroRNAs have been shown to play an important role in insulin signaling but their biological func-
Received 27 January 2014 tion in insulin resistance induced by saturated fatty acids (SFA) remains largely unknown. Here, we
Revised 11 April 2014 report that SFA palmitate and high fat diet (HFD) significantly increase expression of miR-29a in
Accepted 2 May 2014
myocytes. miR-29a targets IRS-1 3’UTR directly and represses IRS-1 expression at the translational
Available online 17 May 2014
level. Furthermore, the ectopic expression of miR-29a impairs insulin signaling and glucose uptake
in myocytes through a substantial decrease in IRS-1. These findings suggest that the up-regulation of
Edited by Laszlo Nagy miR-29a by SFA is causally related to the development of insulin resistance in myocytes.
Ó 2014 Federation of European B