Insulin-like growth factor I enhances collagen synthesis in engineered human tendon tissue.pdf.pdf

Growth Hormone & IGF Research 25 (2015) 13–19
Contents lists available at ScienceDirect
Growth Hormone & IGF Research
journal homepage: ate/ghir
Insulin-like growth factor I enhances collagen synthesis in engineered
human tendon tissue
Andreas Herchenhan ⁎, Monika L. Bayer, Pernilla Eliasson, S. Peter Magnusson, Michael Kjaer
Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Bispebjerg Bakke 23, Building 8, 2nd floor, 2400 Copenhagen, Denmark
Centre for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
article info abstract
Article history: Objective: Isolated human tendon cells form 3D tendon constructs that demonstrate collagen fibrillogenesis and
Received 9 May 2014 feature structural similarities to tendon when cultured under tensile load. The exact role of circulating growth
Received in revised form 6 August 2014 factors for collagen formation in tendon is sparsely examined. We investigated the influence of insulin-like
Accepted 11 September 2014 growth factor I (IGF-I) on tendon construct formation in 3D cell culture.
Available online 22 September 2014
Design: Tendon constructs were grown in or 10% FBS with or without IGF-I (250 mg/ml) supplementation.
Collagen content (fluorometric), mRNA levels (PCR) and fibril diameter (transmission electron microscopy)
Keywords:
were determined at 7, 10, 14, 21