magnetically actuated microrobots as a platform for stem cell transplantation sungwoong jeon参考-匠人.pdf

该【magnetically actuated microrobots as a platform for stem cell transplantation sungwoong jeon参考-匠人 】是由【妙玉】上传分享，文档一共【12】页，该文档可以免费在线阅读，需要了解更多关于【magnetically actuated microrobots as a platform for stem cell transplantation sungwoong jeon参考-匠人 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。:..SCIENCEROBOTICS|RESEARCHARTICLEMEDICALROBOTSCopyright?2019TheAuthors,someicallyactuatedmicrorobotsasaplatformforrightsreserved;exclusivelicenseestemcelltransplantationAmericanAssociationfortheAdvancement1,2341,21,*,SangwonKim*,ShinwonHa,SeungminLee,EunheeKim,,SunHwaPark5,JungHoJeon5,SungWonKim5,6,CheilMoon4,1,2,31,2??4??1,2??,Jin-youngKim,Seong-WoonYu,HongsooChoiicmicrorobotsweredevelopedforthree-dimensionalcultureandtheprecisedeliveryofstemcellsinvitro,exvivo,,oligodendrocytes,,microrobotswereusedtotransportcolorectalcarcinomacancercellstotumormicrotissueinabody-on-a-chip,prisedaninvitroliver-,;themicrorobotstransportedcellstoaStemcelltherapyhasemergedasapromisingmethodforrestoringtargetedareawithinthetransparentyolkofazebrafishembryoandre-ans;forexample,theuseofneuralstemcellsleasedthecellsinvivo(38).icallyactuatedmicrostructurewas(NSCs)orneuralcellsdifferentiatedfromstemcellshasbeenconsideredusedtodelivermesenchymalstemcells(MSCs)intoamicrofluidicforthetreatmentofdegenerativeneuraldisorders,suchasAlzheimer’schannelloadedwithcellsandathree-dimensional(3D)kneejointhttp://robotics./disease(1,2).Numeroustypesofstemcells,whichcanbeembryonic,,orinducedpluripotent,areavailablefromvarioussources(3,4).forarticularcartilageregeneration(39).Inourpreviousstudy(15),weExtensiveprogresshasrecentlybeenmadeinstemcellbiologyandre-culturedanddeliveredhumanembryonickidney293cellsusingcylin-generativemedicine,demonstratingthepotentialofstemcelltechnol-icogyfortreatmentofawidevarietyofdiseases,,themanipulationofthecylindricalandhexa-developmentalandpediatricdiseasestometabolicdiseases,cancer,icpullingusingthegradientfieldandischemia,aswellasage-relateddegenerativediseases(5–7).,(24,38);,ainfluidicenvironmentsandcanbeusedforbiomedicalapplications,icfieldmayinducemoreefficientandcontrollablemo-byguestonMay30,2019suchasneuralinterfacesandcell/drugdelivery(8–13).,forexample,icfields(11–17),Here,weshowthatmicrorobotsfacilitatedtheprecisetargetingofmicrorobotshaveseveraladvantagesasmedicaltreatments,suchasre--ducedpain,riskofinfection,-,comotioninphysiologicalfluidshavebeendeveloped(15,18–36).whichexhibitedrollingandcorkscrewmotionsuponapplicationofaicfield,showedhigherpropulsionefficienciesthanandinvitroenvironments(37–39).,proliferation,-becauseitisharmlessandundergoeslittledistortionorattenuationincroscopy(SEM).-typemicrorobotiscapableofperformingpick-up-themicrorobotstobemovedtoatargetlocationinabody-on-achipand-dropmotionstodeliverolfactoryreceptorneuroncellsadherentto(BoC)ellcultureplatform(invitroliver-tumormicroor-thescaffold-typecapofthemicrorobotonaratbrainslice(37).work),inaventricleofmousebrainslice(exvivo),inaratcubicalandburr-likesphericalmicrorobotsweremanipulatedusingabrainbloodvessel(exvivo),andinalivenudemouse(invivo).Theseresultsdemonstratethefeasibilityofusingmicrorobotsfortargeted1stemcelltransportationandtransplantationinvariousinvitro,exvivo,DepartmentofRoboticsEngineering,DaeguGyeongbukInstituteofScience&(DGIST),Daegu42988,-ETHMicroroboticsRe-searchCenter,DGIST,Daegu42988,(IRIS),ETH,Zurich8092,,DGIST,Daegu42988,-CatholicBiomedicalEngineer-RESULTS6ingInstitute,CollegeofMedicine,TheCatholicUniversityofKorea,Seoul,-HeadandNeckSurgery,’sHospital,TheFabricationandcharacterizationofthemicrorobotsCatholicUniversityofKorea,Seoul06591,-*(MEMS)?-?:******@(.);******@(S.-.);jy.******@(J.-.)(detailsareavailableintheal.,,eaav4317(2019)29May20191of11:..SCIENCEROBOTICS|RESEARCHARTICLE(,EandF,andmovieS1).Theex-icfieldcausedthehelicalmicrorobottorotatealongitslongaxis,-icfield().Thevelocitiesofthemicrorobotsindeionized(DI)waterwerealsoevaluatedwithvariousrotatingfrequencies().Asinglerotationofthehelicalmicrorobotwillpropelitonepitch(80mm)ifthereisnoslippage,(2p×radius=),://robotics./propelledbyacorkscrewmotion,soitcanswimina3Dfluidicenvironment,whereasthesphericalmicrorobotcannotswimina3Dfluidicenvironmentbutcanrollupanddownincurvedhillychannels,suchasbloodvesselsandmi--,wemanipulatedthebyguestonMay30,2019helicalandsphericalmicrorobotstomovethroughverticalandhorizontalmicro-.(A)Schematicofthemicrorobots.(B)Overallenvironment().fabricationprocessofthemicrorobots.(C)CADlayoutsforcylindrical,hexahedral,helical,andsphericalscaffold-typemicrorobots.(D)SEMimagesofthefabricatedcylindrical,hexahedral,helical,,ultureand40mm.(E)Thethreepropulsionmechanismsofthemicrorobots.(F),Figure2showsimmunofluorescence500mm.(G),).,-intoastrocytes,oligodendrocytes,-typemicrorobotsonwhichcellscanbeimagesshowthatdifferentiatedneuronsbecameattachedto,andwereculturedanddifferentiatedin3Dandforwhichtheporositycanbeentangledwith,-(-andmovieS2;).MovieS3showsthographyusingaphotocurablepolymerwithaporesizeof15mm,icmanipulationofthehelicalandsphericalmicrorobotswithwhichwasselectedwithconsiderationtothesizeofthestemcell(,demonstratingthatthemicrorobots1D).patiblebyenabledattachment,proliferation,-TodemonstratethefeasibilityoftargetedcelldeliveryusingthespacewithafieldintensityBandfieldgradient?B(seetheSupple-microrobotsinaBoC,wemanipulatedmicrorobotsloadedwithhumanmentaryMaterials).Thecylindricalandhexahedralmicrorobotswerecolorectalcarcinoma(HCT116)anicfieldgradient(,work,whichisaninvivo–likeinvitrophysiologicalsystem().(15).icfieldinducedcorkscrewingandTheliver-prisedfourlivermicrotissuesrollingmotionsofthehelicalandsphericalmicrorobots,respectively(MTs)umorMTfluidicallyinterconnectedbyamicrofluidical.,,eaav4317(2019)29May20192of11:..SCIENCEROBOTICS|RESEARCHARTICLEDownloadedfromhttp://robotics./byguestonMay30,;differentiatedastrocytes,oligodendrocytes,andneuronsafter72hoursofdifferentiation;andthenumbersofhippocampalNSCsafter24and72hoursofcultureandofdifferentiatedastrocytes,oligodendrocytes,(nestinforNSCs,GFAPforastrocytes,GalCforoligodendrocytes,andTubb3forneurons);Hoechst33342counterstainingwasperformedtovisualizethenuclei(twotypesofmicrorobotswereusedineachexperiment,andeachexperimentwasrepeatedtwotimes).al.,,eaav4317(2019)29May20193of11:..SCIENCEROBOTICS|,transport,orculture().ExvivomanipulationofthemicrorobotsTheexvivomanipulationofthemicro-(AandB,respectively)showsaschematicandphotographoftheexperimentalsetupforexvivomicro--que,sothatthemicrorobotscanbelocal-izedandvisualizedduringmanipulation(40).UsingaHamiltonsyringe(33-),themicrorobotswereinjectedliver-workbybright-field(BF)andCalceinAM–stainedgreenfluorescence(GF)images.(A)Configurationofacell-intotheinternalcarotidartery(ICA),http://robotics./loadedmicrorobot,liver,ansinthemicrofluidicBoC.(B)MicrorobotwithattachedHCT116cellsplacedwhichislocatedbeforethebifurcationinachamber(scalebars,50mm).(C)MovementofamicrorobottothetargettumorMT,passingfourliverMTs(scalebar,oftheanteriorcerebralartery(ACA)and500mm).(D)ArrivalofthemicrorobotatthetargettumorMT(scalebars,50mm).LiverandtumorMTswithacell-loadedmiddlecerebralartery(MCA).Trans-microrobotbefore(E)andafter(F)cultivationfor42hours(scalebars,200mm).parentbloodvesselswerefilledwithDIwater,andthehelicalandsphericalmi--,respectively,(,CandFluorescenceimagingofthecellsattachedonthemicrorobotwascon-DandmovieS6).ThemicrorobotsweremovedfromtheICAtothebyguestonMay30,2019ductedtovisualizetheliveanddeadcellswithgreenandredfluores-ACA(leftbloodvessel)andMCA(rightbloodvessel).Next,toverifycence,,weappliedthemicro-icallydrivenactuationoftherobotstoasliceofmousebraininartificialcerebrospinalfluid(CSF).