longitudinal enlargement of the lesion after spinal cord injury in the rat a consequence of malignant edema p tomko资料.pdf

该【longitudinal enlargement of the lesion after spinal cord injury in the rat a consequence of malignant edema p tomko资料 】是由【四婆子】上传分享，文档一共【9】页，该文档可以免费在线阅读，需要了解更多关于【longitudinal enlargement of the lesion after spinal cord injury in the rat a consequence of malignant edema p tomko资料 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。SpinalCord(2016),1–9&2016InternationalSpinalCordSocietyAllrightsreserved1362-4393/16ORIGINALARTICLELongitudinalenlargementofthelesionafterspinalcordinjuryintherat:aconsequenceofmalignantedema?PTomko1,DFarka?2,D?í?ková1andIVanicky1Studydesign::Quantitativeanalysisofsecondarychangesinlesionsizeafterexperimentalspinalcordinjury(SCI)intherat,::AfterSCIintherat,,thelesionpro??:Immediatelyafterinjury,aspindle-ontusivelesionwasobserved,withthelengthof~,-:?,20September2016;doi:.–9ThisslowevolutionofthelesionInourpreviousexperimentswiththeratSCImodel,ecrosis10–13orevaluatedrecoveryofneurologicalfunctionsduringlong-termsurvival‘autodestruction’.ecrosisresultsinperiods(4–6weeks).1–5Aftersurvival,thelesionsitewashistologicallysecondarylossofvitalspinalcordtissueand,insomespeciesanalyzedinordertoquantitatetheamountofsparedtissueattheincludinghumans,,-,therewereTheprocessesinvolvedinsecondaryinjuryhavebeenextensivelysmalldorsalcolumnlesions(DCLs),occurringbothcraniallyandstudied,,wehaveHowever,?cultiesassociatedwithpoor?,,,,thelesionedtissueisnotIthasbeenrecognizedthat,aftercontusiveSCI,theinjuredtissuesself-supporting,andthepiecesofspinalcordwouldbendanddistortundergoauniqueprocessinwhichthesiteofinjuryisgraduallyduringdehydrationandparaf?nin?,withstandardtransformedintoalarge,cavity-?,tissue-processingtechniques,,alignedspinalshearingandstretchingcausedirecttissuedisruption,,,eventuallycoalescingintothecharacteristicmatrixwas?,injuryinvolvesIthasfacilitatedserialtransversalcuttingandkeptthe?oatingsections1InstituteofNeurobiology,SlovakAcademyofSciences,Ko?ice,Slovakiaand2MedicolegalandPathological-AnatomicalDepartment,HealthcareSurveillanceAuthority,Ko?ice,SlovakiaCorrespondence:DrIVanicky,InstituteofNeurobiology,SlovakAcademyofSciences,Soltesovej4,Ko?ice04001,-mail:******@;revised29June2016;,,sothatthecenterofthemeasurementofthewholelesion(severalcentimeterslong)‘intoto’.balloonwasrestingatT8–°C,theballoonwasrapidlyin?,whichcorrespondstointermediatedegreeofinjury(partialotionwithpermanentneurologicalde?cit).Afterthat,thepressioninjurymodelballoonwasde?atedandthecatheterremoved,?y,%halothaneinroomair,munitymaintainedinhalothaneanesthesia(–2%mitteeattheInstituteofviafacemask)–T10NeurobiologyandtheStateVeterinaryandAlimentaryAdministrationofthespinousprocesses,+(1mmthick)arealignedincolumns(cranialendisleftandup).‘empty’,()-madeAfterSCI,animalswereallowedtosurvivefor5min,1h,1day,2,3,4,5andmoldwithadimension10×?lledwith7days(n=5ineachgroup).Inaddition,twoanimalsweresubjectedtomoreglutaraldehyde-polymerizedproteinmatrixpreparedasfollows:stocksolutionsevereinjury(contusionwith20μlballoon)(150bloom,BDHLtd,Atherstone,Attheendoftheirsurvivalperiod,allanimalsweredeeplyanesthetizedUK),withketamine/xylazineandtranscardiallyperfusedwithsalinefollowedby4%57gofchickeneggalbumin(BDHLtd)?°,9partsofthegelatine–albuminsolutionweremixedwith1post?xedinthesame?-cold25%,,thehardenedmatrixwiththeembeddedspinalTofacilitatesystematicanalysis,wholespinalcordswereembeddedinaproteincordwasremovedfromthemoldandstoredin4%,(TM-100010×10mm2chamberwith1mmslices,ASIInstruments,USA).Figure2SCI+(1mmthick)arealignedincolumns(cranialendisleftandup).,increasednumberofhemorrhagesandprominentcranialandcaudalprotrusionsindorsalcolumns(sections6–23and37–47,respectively).Scalebar,,thedrawingsfromallanimalsinonegroupIndividualslabswerecollectedandstoredinthe?xativeinstandard24--,wewantedtodocumentthedistributionofa?uidwholeslabswere?at-?xedinasterotaxicframe,(50μmdiameter)wasconnectedtoaThelongitudinalsizeofthelesionwasdeterminedasthenumberofsectionsmicrosyringe?lledwith2%(thatis,millimeters),theduramaterwasincisedwithatipofhypodermicparedbytheunpairedStudent’sneedle(400μmlateralfromthemidline),andthemicripipettewasinsertedatt-,ecrosis15°angle(),andthemedio-lateralsizeofthelesionwaspreventleakingoftheinjected?uid,,and10μlofgelatinewasinjectedslowlyfromeachanimalandthelesionshapewasprojectedonthespinalcord(within10min)+(1mmthick)arealignedincolumns(cranialendisleftandup).,,,thetissueswereperfusion-?xedandthespinalcordsAfter3days,,hemorrhagicfocichangedtheircolorfromredtoyellowishand/±,,,anddecolorationwasmorepronounced.?theepicenter,thewholegraymatterareawaslledwithhemorrhage,Inthemostseverelyaffectedareas,,,therewerescatteredAfter7days,therewasfurtherprogressincavitationanddebrissmallhemorrhages,(Figure1).ThecraniocaudalsizeofthewholelesionInaplotsummarizingthechangesinthelesionsizewithinthe?±±(Figure4),itisobviousthatthelesionsizeisexpandingAfter24h,?rst2days,,onlyregressivechangeswereobserved,(Figure5).,theDCLlookedlikea