microbial lysate upregulates host oxytocin bernard j. varian资料.pdf

该【microbial lysate upregulates host oxytocin bernard j. varian资料 】是由【四婆子】上传分享，文档一共【58】页，该文档可以免费在线阅读，需要了解更多关于【microbial lysate upregulates host oxytocin bernard j. varian资料 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。:..AcceptedManuscriptFull-,TheofilosPoutahidis,,TatianaLevkovich,YassinIbrahim,EliskaDidyk,LanaShikhman,,AlexandrosHardas,,KumaranKolandaivelu,,,:S0889-1591(16)30492-5DOI:http://dx./.:YBRBI3004Toappearin:Brain,Behavior,andImmunityReceivedDate:14August2016RevisedDate:17October2016AcceptedDate:3November2016Pleasecitethisarticleas:Varian,.,Poutahidis,T.,DiBenedictis,.,Levkovich,T.,Ibrahim,Y.,Didyk,E.,Shikhman,L.,Cheung,.,Hardas,A.,iardi,.,Kolandaivelu,K.,Veenema,.,Alm,.,Erdman,.,Microbiallysateupregulateshostoxytocin,Brain,Behavior,andImmunity(2016),doi:http://dx./.,typesetting,,andalllegaldisclaimersthatapplytothejournalpertain.:..1Microbiallysateupregulateshostoxytocin21*1,2*,TheofilosPoutahidis,,Tatiana111,454Levkovich,YassinIbrahim,EliskaDidyk,LanaShikhman,,AlexandrosHardas,iardi,Kumaran634,7,81**6Kolandaivelu,,EricJAlm,,MassachusettsInstituteofTechnology,Cambridge,,Thessaloniki,,DepartmentofPsychology,BostonCollege,12ChestnutHill,,MassachusettsInstituteofTechnology,Cambridge,,Worcester,,InstituteforMedicalEngineering&Science,Massachusetts17InstituteorTechnology,Cambridge,,MassachusettsInstituteofTechnology,Cambridge,,Cambridge,MAUSA2122*firstauthorscontributedequally23**correspondingauthor242526ABSTRACT2728Neuropeptidehormoneoxytocinhasrolesinsocialbonding,energymetabolism,29andwoundhealingcontributingtogoodphysical,()issufficienttoup--,-producing1:..1cellswerefoundtobeincreasedinthecaudalparaventricularnucleus[PVN],3coincidentwithloweredbloodlevelsofstresshormonecorticosteroneandmore4rapidepidermalclosure,:bacteria,postbiotic,stress,corticosterone,thymus,woundhealing1011Runningheader:Microbesregulateoxytocin1213INTRODUCTION1415Inaseriesofpriorstudiesusingmicewehaveshownthattheprototypeprobiotic16bacteriumLactobacillusreuteri()impartsanarrayofhealthbenefits17(ErdmanandPoutahidis,2014;Ibrahim,2014;Lakritzetal.,2014;Levkovichetal.,182013;Poutahidisetal.,2013a;Poutahidisetal.,2013b;Poutahidisetal.,2014;19Poutahidisetal.,2015;al.,2016a;al.,2016b;al.,202014).Asidefromitspreviouslydescribedpositivecontributionsintheheathof21oral(Iniestaetal.,2012;Martin-Cabezasetal.,2016)andgastrointestinal(GI)22tractmucosa(Cruchetetal.,2015;Gaoetal.,2015;Liuetal.,2012;Schreiberet23al.,2009;al.,2016a;Walteretal.,2011),theprobiotichassystemic24effectsontheimmuneandhormonalprofileofthehostanimal(al.,252016;ErdmanandPoutahidis,2014;Ibrahim,2014;Lakritzetal.,2014;Leeetal.,262016;Levkovichetal.,2013;al.,2010;Poutahidisetal.,2013a;2:..1Poutahidisetal.,2013b;Poutahidisetal.,2014;Poutahidisetal.,2015;2al.,2015;al.,2016a;al.,2016b;al.,2014).(Ibrahim,2014;al.,2016a)andresist4obesity(FakandBackhed,2012;Poutahidisetal.,2013b;Qiaoetal.,2015;Varian5etal.,2016b;al.,2014)andage-hangesofskeletal6muscle(al.,2016a),testis(Poutahidisetal.,2014),andthyroid(7al.,2014)andthymusglands(al.,2016a).Theyalsohavehealthyfur8coats(ErdmanandPoutahidis,2014;Levkovichetal.,2013),healtheirskin9woundsfaster(Poutahidisetal.,2013a)andareresistanttointestinal(10al.,2016a),mammarygland,liver,andlungcancer(Lakritzetal.,2014;Poutahidis11etal.,2015).(Ibrahim,2014),wediscovered15thattheprobioticincreasestheplasmalevelsofoxytocin(Ibrahim,2014;16Poutahidisetal.,2013a;al.,2016b).(ErdmanandPoutahidis,2014;Ibrahim,2014;Poutahidisetal.,202013a;al.,2016b).Interestingly,-,(al.,2014;Collinsetal.,2016;Poutahidisetal.,232013a;Poutahidisetal.,2013b;al.,2016a;al.,2016b;Varian24etal.,2014;Zhangetal.,2015b),theexogenousadministrationofoxytocinhaseleratewoundhealing(Gavrilenkoetal.,2003;al.,3:..12010;Vitaloetal.,2009),counteractobesity(Barengolts,2016;BlevinsandBaskin,22015),suppressuncontrolledinflammation(Wangetal.,2015)andprevent3musclewasting(Costaetal.,2014;Elabdetal.,2014)andboneloss(Colaianniet4al.,2014;al.,2016;Tammaetal.,2009).56Aroleforoxytocininpromotinggoodmentalhealth,includingsocialbonding,a7generalsenseofwell-being,andimprovedlearningandmemoryisemerging8(Carter,2014;DonaldsonandYoung,2008;al.,2016).Atthesame9time,recentevidencesupportingthepresenceofagut-microbiota-brainaxis10(al.,2013;Kellyetal.,2016;al.,2016)suggeststhatprobiotic11bacteriacaninfluenceemotions,mood,anxietyanddepression(al.,122013;Kellyetal.,2016;al.,2016).Thisplacesoxytocin(Carter,2014;13DonaldsonandYoung,2008;al.,2016)andprobioticbacteriaamong14novelapproachesforpreventingoreventreatingneuropsychiatricdisorders15(al.,2013;Kellyetal.,2016;al.,2016).Hence,theirname16psychobiotics(al.,2013).(Ibrahim,2014;Poutahidisetal.,2013a;al.,202016b),untilrecentlytherewerenootherdatasuggestingthatthisbacterium21couldbeconsideredamongsuspectedpsychobiotics(al.,2013).However,22arecentstudyinmice(al.,2016)supportsthisnotionandexpands23priorfindingsconnectingmaternalobesity-,4:..,stimulatesthemesolimbicdopaminerewardsystemand3promotesprosocialbehavior(al.,2016).(Poutahidisetal.,2015).67Amongthenewlyrecognizedactionsofoxytocin,itsconnectionwiththeimmune8system,isparticularlyimportant(Wangetal.,2015).Indeed,oxytocincontributes9tothematurationandselectionofT-lymphocytesinthethymus(Hansenneetal.,102009;Hansenneetal.,2005;Wangetal.,2015),-inducedupregulationoftheForkheadBoxN1(FoxN1)14inthymicepithelialcells(al.,2016a).FoxN1isessentialfornormalpetenthostimmunesystemasexemplifiedby16micedeficientinFoxN1(udemice),whichlackfunctionalT-lymphocytes17(Nehlsetal.,1994;Romanoetal.,2013;Zhangetal.,2012),-establishedeffectofoxytocinintheimmunesystemrelateswithits21abilitytodown-regulateneutrophilicpro-inflammatoryresponses(Al-Amranand22Shahkolahi,2013;Biyiklietal.,2006;Iserietal.,2005a,b;al.,2001;23Wangetal.,2015).(Poutahidisetal.,2013a;5:..al.,2016a;al.,2016b).,metabolic6disordersandcardiovasculardisease(CoffeltSB,2016;FC,2016;Manda-Handzlik7andDemkow,2015;Seijkensetal.,2014).However,whileoxytocinhasapparent8beneficialeffects,(DeDreuetal.,2011).Also,inpatientssufferingfrom12borderlinepersonalitydisorder,oxytocindecreasedtrustandcooperation(Bartz13etal.,2011).1415Giventhechallengesraisedregardingtheefficacyandsafetyofexogenous16oxytocinadministration(LefevreandSirigu,2016;LengandLudwig,2016),,includingoxytocininductionandeventuallytherapeutic20strategies,however,-regulationofoxytocinandassociatedhealthbenefits?2223Inthepresentstudy,:..1improvementsandincreasesthenumberofoxytocin-positivecellsinthecaudal2portionoftheparaventricularnucleusofthehypothalamus(PVN)--5inducedoxytocinisnecessaryfortheinductionofimportantbeneficialhost6effects,,-regulationofoxytocinandhealth8benefitsinmice,suggestingthatthesephenomenaaretriggeredbyabacterial9component,:FemaleoutbredSwissstockCD-1femalemice(CharlesRiver,14WilmingtonMA),C57BL/6wildtype(wt),oxytocin-wt[ot-wt]andoxytocin-15knockout(ot-ko)B6;129S-Oxttm1Wsy/Jmice(purchasedinitiallyfromJacksonlabs;16BarHarbor,ME)(AAALAC)-'s(MIT)mitteeonAnimalCare(CAC).Micewerehousedunderstandard12:-,andgrouphousedwith4-5miceper7:..-.-3PTA-:Ineachexperiment,subsetsofmice6receivedintheirdrinkingwaterastrainofL.-PTA-6475cultivatedas7describedelsewhere(Poutahidisetal.,2013a;Saulnieretal.,2011).-