TABLE S1.Strains and plasmids used in this study.doc

TABLE S1. Strains and plasmids used in this study
Strain or plasmid
Description
Source or reference
Strains
B. pertussis
Tohama I
B. bronchiseptica
RB50
RB50ΔBB2692
RB50ΔBB2752
RB50ΔBB3521
RB50ΔBB3522
RB50ΔBB4782

E. coli
HB101
DH5α
JM109
BL21(DE3)
DH5αλpir
Plasmids
pCACT3
pRK2013
pBBR1MCS-5
pKK232-8
pET-28b
pABB-CRS2
pABB-CRS2-Gm
pIVET-IP vector
pIVET-IP-PBB0324
pET-28b-BBFtsZ
pBBr01-SD vector
pBBr01-SD-BB2692
pBBr01-SD-BB2752
pBBr01-SD-BB3521
pBBr01-SD-BB3522
pBBr01-SD-BB4782
Wild type
Wild type
RB50 derivative
RB50 derivative
RB50 derivative
RB50 derivative
RB50 derivative
K-12 cloning strain
K-12 cloning strain
K-12 cloning strain
K-12 cloning strain
K-12 cloning strain of plasmid with R6K origin
Apr, plasmid carrying cyaA and cyaC genes from B. pertussis
Kmr, helper plasmid containing tra and mob genes
Gmr, broad-host-range cloning vector
Cmr, cloning vector
Kmr, expression vector carrying His tag sequences
Apr, R6K-derived suicide vector
Gmr, R6K-derived suicide vector
pBBR1MCS-5 derivative containing rrnBT1T2, cloning site, c-myc-tagged brkA
pIVET-IP derivative containing BB0324 promoter region
pET-28b derivative carrying His-tagged ftsZ (BB4193)
pBBR1MCS-5 derivative containing BBr01 promoter, cloning site, Shine-Dalgarno sequence
pBBr01-SD derivative containing BB2692
pBBr01