胰酶的絮凝沉淀分离分析.docx

硼川火学硕士学位论文
胰酶的絮凝沉淀分离研究
生物化工专业研究生唐爱星 指导老师何泽趋
摘要
胰酶是从猪、牛、羊等胰脏中提取的多种酶的混合物,是我国生化制药行业的重要产品之一。本论文研究了絮凝法沉淀分离胰酶。通过实验,筛选出了具有良好絮凝效果的絮凝剂——壳聚糖。探讨了壳聚糖用量、pH值、温度等因素埘絮凝作用的影响,并比较了絮凝胰酶和***胰酶性质的差异。
壳聚糖的用量、pH值和温度对絮凝有明显的影响。增加壳聚糖的用量有利 r絮凝沉淀,但是会造成胰酶比酶活的下降,实验表明壳聚糖的适宜用量为 ;在pH5一-6范围内,絮凝效果好,胰酶比酶活相对较高:升高温度有利于絮凝但会造成酶活损失,故一般选择在20℃左右操作。
用欧洲药店标准Jv方法测定絮凝胰酶的比酶活,结果为蛋白酶1307U/g、、,酶活收率分别为:%、%1N %。其中蛋白酶和淀粉酶的活力较低,与***胰酶(3159U/)相比有较大的差距,但是脂肪酶活力较高,高于***。测定絮凝沉淀胰酶滤液的酶活,结果滤液中酶活所占的比率很低,说明絮凝沉淀比较有效。
经测定,絮凝胰酶的热稳定性显著增强,最适温度比***胰酶提高10。C; 最适pH值向酸性方向移动,对酸性环境的稳定性增)Jib表观米氏常数K。有所增加。说明壳聚糖对胰酶有固定化作用。
关键词:胰酶,絮凝,壳聚糖,分离
四川大学硕士学位论文
Study on the Separation of Pancreatin with ulation
Major:Biochemical Engineering Graduate:Tang Aixing Supervisor: Zechao
Abstract
Pancreatin iS a mixture of variOtiS enzymes that iS extracted from pancreas of pig,COW,sheep,etc—It is one of the important products of OLlr county in bio—chemical medicine of pancreatin with ulation was studied in this as a good ulant Was found out after experiments.
The influence of the addition of chitosan,the pH,and the temperature WaS evaluated. And the properties of pancreatin separating with ulation pared with pancreatin separating witll acetone.
The addition of chitosan,the pH,and the temperature had obvious inHuence to the addition of chitosan Was advantage of ulmion,but would lead to the activity of enzyme descend,SO a feaSible addition of chitosan was %of protein;within the scopes of pH5-6,the ulation was effective,and the specific activity of enzyme Was relatively higher;rising the temperature was advantage of ulation,but would result in enzyme deactivation,SO the optimum temperature for operation Was 20*(2.
The specific activities of pancreatin separating with ulation were protease 1307U/g,lipase 74,65kU/g and amylase ,and the yields ofactivity were 1 %,%and %,,the specific activities of